Anti-ATP6V0A2 antibody (ab96803)
Key features and details
- Rabbit polyclonal to ATP6V0A2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ATP6V0A2 antibody -
Description
Rabbit polyclonal to ATP6V0A2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment corresponding to a region within amino acids 156-404 of Human ATP6V0A2 (NP_036595).
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Positive control
- 293T, A431 and H1299 whole cell lysates; DLD1 xenograft; HeLa, HepG2, MOLT4 and Raji cell lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96803 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 98 kDa.
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IHC-P |
1/100 - 1/500.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 98 kDa. |
IHC-P
1/100 - 1/500. |
Target
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Function
Part of the proton channel of V-ATPases. Essential component of the endosomal pH-sensing machinery. May play a role in maintaining the Golgi functions, such as glycosylation maturation, by controlling the Golgi pH. -
Involvement in disease
Defects in ATP6V0A2 are the cause of cutis laxa autosomal recessive type 2A (ARCL2A) [MIM:219200]. An autosomal recessive disorder characterized by an excessive congenital skin wrinkling, a large fontanelle with delayed closure, a typical facial appearance with downslanting palpebral fissures, a general connective tissue weakness, and varying degrees of growth and developmental delay and neurological abnormalities. Some affected individuals develop seizures and mental deterioration later in life, whereas the skin phenotype tends to become milder with age. At the molecular level, an abnormal glycosylation of serum proteins is observed in many cases.
Defects in ATP6V0A2 are a cause of wrinkly skin syndrome (WSS) [MIM:278250]. WSS is rare autosomal recessive disorder characterized by wrinkling of the skin of the dorsum of the hands and feet, an increased number of palmar and plantar creases, wrinkled abdominal skin, multiple musculoskeletal abnormalities, microcephaly, growth failure and developmental delay. -
Sequence similarities
Belongs to the V-ATPase 116 kDa subunit family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cell membrane. Endosome membrane. In kidney proximal tubules, also detected in subapical vesicles. - Information by UniProt
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Database links
- Entrez Gene: 338038 Cow
- Entrez Gene: 23545 Human
- Entrez Gene: 21871 Mouse
- Entrez Gene: 116455 Rat
- Omim: 611716 Human
- SwissProt: O97681 Cow
- SwissProt: Q9Y487 Human
- SwissProt: P15920 Mouse
see all -
Alternative names
- a2 antibody
- A2V ATPase antibody
- ARCL antibody
see all
Images
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All lanes : Anti-ATP6V0A2 antibody (ab96803) at 1/10000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 98 kDa
7.5% SDS PAGE -
ab96803, at 1/500 dilution, staining ATP6V0A2 in paraffin-embedded DLD1 xenograft by Immunohistochemistry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab96803 has been referenced in 5 publications.
- Chitirala P et al. Role of V-ATPase a3-Subunit in Mouse CTL Function. J Immunol 204:2818-2828 (2020). PubMed: 32269094
- Rivera OC et al. A common genetic variant in zinc transporter ZnT2 (Thr288Ser) is present in women with low milk volume and alters lysosome function and cell energetics. Am J Physiol Cell Physiol 318:C1166-C1177 (2020). PubMed: 32320289
- Kissing S et al. Disruption of the vacuolar-type H+-ATPase complex in liver causes MTORC1-independent accumulation of autophagic vacuoles and lysosomes. Autophagy 13:670-685 (2017). PubMed: 28129027
- Tripathi M et al. Hyperhomocysteinemia causes ER stress and impaired autophagy that is reversed by Vitamin B supplementation. Cell Death Dis 7:e2513 (2016). PubMed: 27929536
- Kissing S et al. Vacuolar ATPase in phagosome-lysosome fusion. J Biol Chem 290:14166-80 (2015). PubMed: 25903133