Overview

  • Product name
    Anti-ATP6V0C antibody
  • Description
    Rabbit polyclonal to ATP6V0C
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Sheep, Rabbit, Horse, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 71-120 (VVAVLIANSL NDDISLYKSF LQLGAGLSVG LSGLAAGFAI GIVGDAGVRG) of Human ATP6V0C (NP_001685)

  • Positive control
    • OVCAR3 cell lysate This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MCF-7.

Properties

Applications

Our Abpromise guarantee covers the use of ab104374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 16 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Relevance
    ATP6V0C is a component of the V0 domain (proton pore complex) of vacuolar ATPase (V-ATPase). Vacuolar ATPase is a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation.
  • Cellular localization
    Vacuole membrane; Multi-pass membrane protein.
  • Database links
  • Alternative names
    • ATP6C antibody
    • ATP6L antibody
    • ATPase, H+ transporting, lysosomal, V0 subunit c antibody
    • ATPL antibody
    • V ATPase subunit c antibody
    • Vacuolar ATP synthase 16 kDa proteolipid subunit antibody
    • Vacuolar H+ ATPase proton channel subunit antibody
    • Vacuolar proton pump, 16 kDa subunit antibody
    • VATL antibody
    • Vma3 antibody
    see all

Images



  • Predicted band size : 16 kDa
    Western blot analysis of ACHN cell lysate labeling ATP6V0C with ab104374 at 1.0µg/ml.
  • ICC/IF image of ab104374 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab104374 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-ATP6V0C antibody (ab104374) at 1 µg/ml + OVCAR3 cell lysate at 10 µg

    Predicted band size : 16 kDa
    Gel concentration 12%

References

This product has been referenced in:
  • Milkereit R  et al. LAPTM4b recruits the LAT1-4F2hc Leu transporter to lysosomes and promotes mTORC1 activation. Nat Commun 6:7250 (2015). Read more (PubMed: 25998567) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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