Customer reviews and FAQs

Overview

  • Product name
    Anti-ATP6V0D1 antibody
    See all ATP6V0D1 primary antibodies
  • Description
    Mouse monoclonal to ATP6V0D1
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: AKLFPHCGRL YPEGLAQLAR ADDYEQVKNV ADYYPEYKLL FEGAGSNPGD KTLEDRFFEH EVKLNKLAFL N, corresponding to amino acids 238-309 of Human ATP6V0D1

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

Applications

Our Abpromise guarantee covers the use of ab56441 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 40 kDa.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Subunit of the integral membrane V0 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. May play a role in coupling of proton transport and ATP hydrolysis (By similarity). May play a role in cilium biogenesis through regulation of the transport and the localization of proteins to the cilium.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the V-ATPase V0D/AC39 subunit family.
  • Cellular localization
    Membrane. Localizes to centrosome and the base of the cilium.
  • Information by UniProt
  • Database links
    • Alternative names
      • 32 kDa accessory protein antibody
      • ATP6D antibody
      • ATP6DV antibody
      • ATP6V0D1 antibody
      • ATPase H+ transporting lysosomal (vacuolar proton pump) member D antibody
      • ATPase H+ transporting lysosomal 38kD V0 subunit d antibody
      • ATPase H+ transporting lysosomal 38kDa V0 subunit d1 antibody
      • ATPase H+ transporting lysosomal V0 subunit d1 antibody
      • H(+) transporting two sector ATPase subunit D antibody
      • p39 antibody
      • V ATPase 40 KDa accessory protein antibody
      • V ATPase AC39 subunit antibody
      • V ATPase subunit d 1 antibody
      • V ATPase subunit D antibody
      • V-ATPase 40 kDa accessory protein antibody
      • V-ATPase AC39 subunit antibody
      • V-ATPase subunit d 1 antibody
      • V-type proton ATPase subunit d 1 antibody
      • VA0D1_HUMAN antibody
      • Vacuolar ATP synthase subunit d 1 antibody
      • Vacuolar proton pump subunit d 1 antibody
      • VATX antibody
      • VMA 6 antibody
      • VMA6 antibody
      • VPATPD antibody
      see all

    Anti-ATP6V0D1 antibody images

    • IHC-P - ATP6V0D1 antibody (ab56441)
      IHC-P - ATP6V0D1 antibody (ab56441)
      ATP6V0D1 antibody (ab56441) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human stomach.
    • Western blot - ATP6V0D1 antibody (ab56441)
      Western blot - ATP6V0D1 antibody (ab56441)


      Predicted band size : 40 kDa
      ATP6V0D1 antibody (ab56441) at 1ug/lane + HeLa cell lysate at 25ug/lane.
    • Immunocytochemistry/ Immunofluorescence-ATP6V0D1 antibody(ab56441)
      Immunocytochemistry/ Immunofluorescence-ATP6V0D1 antibody(ab56441)
      ICC/IF image of ab56441 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56441, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Flow Cytometry - ATP6V0D1 antibody (ab56441)
      Flow Cytometry - ATP6V0D1 antibody (ab56441)

      Overlay histogram showing HeLa cells stained with ab56441 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56441, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was a mix of mouse IgG1 [ICIGG1], (ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4%PFA/permeabilized in 0.1% PBS-Tween used under the same conditions.

    References for Anti-ATP6V0D1 antibody (ab56441)

    This product has been referenced in:
    • Matsuda S  et al. A cytotoxic type III secretion effector of Vibrio parahaemolyticus targets vacuolar H+-ATPase subunit c and ruptures host cell lysosomes. PLoS Pathog 8:e1002803 (2012). WB . Read more (PubMed: 22829766) »
    • Taguwa S  et al. Dysfunction of autophagy participates in vacuole formation and cell death in cells replicating hepatitis C virus. J Virol 85:13185-94 (2011). Read more (PubMed: 21994453) »

    See all 2 Publications for this product

    Publishing research using ab56441? Please let us know so that we can cite the reference in this datasheet.

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    2 Abreviews or Q&A

    10
    Votes: Highest First

    Western blot abreview for Anti-ATP6V0D1 antibody

     Excellent
    Abreviews
    Application
    Western blot
    Sample
    Pig Cell lysate - other (LLC-PK1)
    Gel Running Conditions
    Non-reduced Denaturing (4-15%)
    Loading amount
    10 µg
    Specification
    LLC-PK1
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 1.5% · Temperature: 21°C
    Read More
    abreview image
    Username

    Dr. Christina Mcguire

    Verified customer

    Submitted Dec 22 2015

    Customer kindly contacted us as they were unable to see any signal when using the product in the following conditions:

    Sample:HeLa cells.  4% formaldehyde fixed (10 min)

    Blocking and Permeablization: 1%BSA / 10% normal goat serum ...

    Read More

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxxx.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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