- Datasheet
- Specific References (2)
- Protocols
Overview
- Product nameAnti-ATP6V0D1 antibody
See all ATP6V0D1 primary antibodies - DescriptionMouse monoclonal to ATP6V0D1
- Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
- Species reactivityReacts with: Human
- Immunogen
Recombinant fragment: AKLFPHCGRL YPEGLAQLAR ADDYEQVKNV ADYYPEYKLL FEGAGSNPGD KTLEDRFFEH EVKLNKLAFL N, corresponding to amino acids 238-309 of Human ATP6V0D1
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
PBS, pH 7.2 -
Concentration information loading... - PurityProtein G purified
- ClonalityMonoclonal
- IsotypeIgG1
- Light chain typekappa
- Research areas
Associated products
- Compatible Secondaries
- Isotype control
Applications
Our Abpromise guarantee covers the use of ab56441 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 40 kDa. | |
| IHC-P | Use a concentration of 5 µg/ml. | |
| ICC/IF | Use a concentration of 10 µg/ml. | |
| Flow Cyt | Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
- FunctionSubunit of the integral membrane V0 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. May play a role in coupling of proton transport and ATP hydrolysis (By similarity). May play a role in cilium biogenesis through regulation of the transport and the localization of proteins to the cilium.
- Tissue specificityUbiquitous.
- Sequence similaritiesBelongs to the V-ATPase V0D/AC39 subunit family.
- Cellular localizationMembrane. Localizes to centrosome and the base of the cilium.
- Information by UniProt
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Database links
- Entrez Gene: 9114 Human
- Omim: 607028 Human
- SwissProt: P61421 Human
- Unigene: 106876 Human
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Alternative names
- 32 kDa accessory protein antibody
- ATP6D antibody
- ATP6DV antibody
see all
Anti-ATP6V0D1 antibody images
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IHC-P - ATP6V0D1 antibody (ab56441)ATP6V0D1 antibody (ab56441) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human stomach. -
Western blot - ATP6V0D1 antibody (ab56441)
Predicted band size : 40 kDa
ATP6V0D1 antibody (ab56441) at 1ug/lane + HeLa cell lysate at 25ug/lane. -
Immunocytochemistry/ Immunofluorescence-ATP6V0D1 antibody(ab56441)ICC/IF image of ab56441 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56441, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Flow Cytometry - ATP6V0D1 antibody (ab56441)Overlay histogram showing HeLa cells stained with ab56441 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56441, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was a mix of mouse IgG1 [ICIGG1], (ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4%PFA/permeabilized in 0.1% PBS-Tween used under the same conditions.
References for Anti-ATP6V0D1 antibody (ab56441)
This product has been referenced in:
- Matsuda S et al. A cytotoxic type III secretion effector of Vibrio parahaemolyticus targets vacuolar H+-ATPase subunit c and ruptures host cell lysosomes. PLoS Pathog 8:e1002803 (2012). WB . Read more (PubMed: 22829766) »
- Taguwa S et al. Dysfunction of autophagy participates in vacuole formation and cell death in cells replicating hepatitis C virus. J Virol 85:13185-94 (2011). Read more (PubMed: 21994453) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"