- Datasheet
- Specific References (5)
- Protocols
Overview
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Product nameAnti-ATP6V0D1 antibody
See all ATP6V0D1 primary antibodies -
DescriptionMouse monoclonal to ATP6V0D1
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Host speciesMouse
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Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
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Species reactivityReacts with: Human
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Immunogen
Recombinant fragment: AKLFPHCGRL YPEGLAQLAR ADDYEQVKNV ADYYPEYKLL FEGAGSNPGD KTLEDRFFEH EVKLNKLAFL N, corresponding to amino acids 238-309 of Human ATP6V0D1
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General notes
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab202899 as a replacement.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: None
PBS, pH 7.2 -
Concentration information loading... -
PurityProtein G purified
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ClonalityMonoclonal
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IsotypeIgG1
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Light chain typekappa
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab56441 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 40 kDa. | |
| IHC-P | Use a concentration of 5 µg/ml. | |
| ICC/IF | Use a concentration of 10 µg/ml. | |
| Flow Cyt | Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Target
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FunctionSubunit of the integral membrane V0 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. May play a role in coupling of proton transport and ATP hydrolysis (By similarity). May play a role in cilium biogenesis through regulation of the transport and the localization of proteins to the cilium.
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Tissue specificityUbiquitous.
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Sequence similaritiesBelongs to the V-ATPase V0D/AC39 subunit family.
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Cellular localizationMembrane. Localizes to centrosome and the base of the cilium.
- Information by UniProt
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Database links
- Entrez Gene: 9114 Human
- Omim: 607028 Human
- SwissProt: P61421 Human
- Unigene: 106876 Human
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Alternative names
- 32 kDa accessory protein antibody
- ATP6D antibody
- ATP6DV antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V0D1 antibody (ab56441)ATP6V0D1 antibody (ab56441) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human stomach. -
Western blot - Anti-ATP6V0D1 antibody (ab56441)ATP6V0D1 antibody (ab56441) at 1ug/lane + HeLa cell lysate at 25ug/lane. -
Immunocytochemistry/ Immunofluorescence - Anti-ATP6V0D1 antibody (ab56441)ICC/IF image of ab56441 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56441, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Flow Cytometry - Anti-ATP6V0D1 antibody (ab56441)Overlay histogram showing HeLa cells stained with ab56441 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56441, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was a mix of mouse IgG1 [ICIGG1], (ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4%PFA/permeabilized in 0.1% PBS-Tween used under the same conditions.
Protocols
References
This product has been referenced in:
- Jacquin E et al. Pharmacological modulators of autophagy activate a parallel noncanonical pathway driving unconventional LC3 lipidation. Autophagy 13:854-867 (2017). Read more (PubMed: 28296541) »
- Florey O et al. V-ATPase and osmotic imbalances activate endolysosomal LC3 lipidation. Autophagy 11:88-99 (2015). Read more (PubMed: 25484071) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"