Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab56556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceVacuolar ATPase (V ATPase) is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'' and d). It is responsible for acidifying a variety of intracellular organelles in eukaryotic cells. ATP6V0D2 is a component of the integral membrane V0 complex of vacuolar ATPase and may have a role in coupling of proton transport and ATP hydrolysis. ATP6V0D2 may be involved in regulation of osteoclast fusion and bone formation.
    • Cellular localizationApical plasma membrane
    • Database links
    • Alternative names
      • ATP6D2 antibody
      • ATPase H+ transporting lysosomal 38kDa V0 subunit D antibody
      • ATPase H+ transporting lysosomal 38kDa V0 subunit D isoform 2 antibody
      • ATPase H+ transporting lysosomal 38kDa V0 subunit D2 antibody
      • FLJ38708 antibody
      • V ATPase subunit d 2 antibody
      • Vacuolar ATP synthase subunit d 2 antibody
      • Vacuolar proton pump subunit d 2 antibody
      • VMA 6 antibody
      • VMA6 antibody
      see all

    Anti-ATP6V0D2 antibody images

    • Western blot against tagged recombinant protein immunogen using ab56556 ATP6V0D2 antibody at 1ug/ml. Predicted band size of immunogen is 34 kDa

    References for Anti-ATP6V0D2 antibody (ab56556)

    ab56556 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Dog Cell lysate - whole cell (Kidney cells)
    Loading amount 20 µg
    Specification Kidney cells
    Treatment Treat as well as non-treated
    Gel Running Conditions Non-reduced Denaturing (12% gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

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    Verified customer

    Submitted Jul 22 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"