Recombinant
RabMAb

Anti-ATP6V1D antibody [EPR11326(B)] (ab157458)

Overview

  • Product name
    Anti-ATP6V1D antibody [EPR11326(B)]
  • Description
    Rabbit monoclonal [EPR11326(B)] to ATP6V1D
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human ATP6V1D (Uniprot: Q9Y5K8).

  • Positive control
    • Human breast and kidney tissues; Human fetal brain, Y79, 293T and HeLa cell lysates; HeLa and 293T cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab157458 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 28 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Relevance
    ATP6V1D is a subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'' and d). It is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system.
  • Cellular localization
    Cell Membrane and Cytoplasmic
  • Database links
  • Alternative names
    • ATP6M antibody
    • ATP6V1D antibody
    • V-ATPase 28 kDa accessory protein antibody
    • V-ATPase subunit D antibody
    • V-type proton ATPase subunit D antibody
    • Vacuolar ATP synthase subunit D antibody
    • Vacuolar proton pump subunit D antibody
    • VATD antibody
    • VMA8 antibody
    see all

Images

  • All lanes : Anti-ATP6V1D antibody [EPR11326(B)] (ab157458) at 1/10000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Y79 cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 28 kDa
  • Immunohistochemical analysis of Paraffin-embedded Human breast tissue labeling ATP6V1D with ab157458 at 1/50 dilution.
  • Immunohistochemical analysis of Paraffin-embedded Human kidney tissue labeling ATP6V1D with ab157458 at 1/50 dilution
  • Flow cytometric analysis of permeabilized 293T cells labeling ATP6V1D with ab157458 at 1/10 dilution (red) or a rabbit IgG negative (green).
  • Immunofluorescent analysis of HeLa cells labeling ATP6V1D with ab157458 at 1/100 dilution.
  • Detection of ATP6V1D by Western Blot of Immunprecipitate. Y79 cell lysate immunoprecipitated using ab157458 at 1/10 dilution.

References

ab157458 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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