Anti-ATP6V1D antibody [EPR11326(B)] (ab157458)
- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-ATP6V1D antibody [EPR11326(B)]
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DescriptionRabbit monoclonal [EPR11326(B)] to ATP6V1D
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide, corresponding to residues in Human ATP6V1D (Uniprot: Q9Y5K8).
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Positive control
- Human breast and kidney tissues; Human fetal brain, Y79, 293T and HeLa cell lysates; HeLa and 293T cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20ºC.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR11326(B)
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IsotypeIgG
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Research areas
Associated products
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab157458 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Predicted molecular weight: 28 kDa. | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/250. | |
| IP | 1/10 - 1/100. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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RelevanceATP6V1D is a subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'' and d). It is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system.
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Cellular localizationCell Membrane and Cytoplasmic
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Database links
- Entrez Gene: 51382 Human
- Entrez Gene: 73834 Mouse
- Entrez Gene: 299159 Rat
- SwissProt: Q9Y5K8 Human
- SwissProt: P57746 Mouse
- Unigene: 272630 Human
- Unigene: 311549 Mouse
- Unigene: 106041 Rat
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Alternative names
- ATP6M antibody
- ATP6V1D antibody
- V-ATPase 28 kDa accessory protein antibody
see all
Images
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![Western blot - Anti-ATP6V1D antibody [EPR11326(B)] (ab157458)](http://a.abcam.com/ps/datasheet/images/157/ab157458/ATP6V1D-Primary-antibodies-ab157458-2.jpg)
Western blot - Anti-ATP6V1D antibody [EPR11326(B)] (ab157458)All lanes : Anti-ATP6V1D antibody [EPR11326(B)] (ab157458) at 1/10000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Y79 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 28 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1D antibody [EPR11326(B)] (ab157458)](http://a.abcam.com/ps/datasheet/images/157/ab157458/ATP6V1D-Primary-antibodies-ab157458-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1D antibody [EPR11326(B)] (ab157458)Immunohistochemical analysis of Paraffin-embedded Human breast tissue labeling ATP6V1D with ab157458 at 1/50 dilution. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1D antibody [EPR11326(B)] (ab157458)](http://a.abcam.com/ps/datasheet/images/157/ab157458/ATP6V1D-Primary-antibodies-ab157458-4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1D antibody [EPR11326(B)] (ab157458)Immunohistochemical analysis of Paraffin-embedded Human kidney tissue labeling ATP6V1D with ab157458 at 1/50 dilution -
![Flow Cytometry - Anti-ATP6V1D antibody [EPR11326(B)] (ab157458)](http://a.abcam.com/ps/datasheet/images/157/ab157458/ATP6V1D-Primary-antibodies-ab157458-5.jpg)
Flow Cytometry - Anti-ATP6V1D antibody [EPR11326(B)] (ab157458)Flow cytometric analysis of permeabilized 293T cells labeling ATP6V1D with ab157458 at 1/10 dilution (red) or a rabbit IgG negative (green). -
![Immunocytochemistry/ Immunofluorescence - Anti-ATP6V1D antibody [EPR11326(B)] (ab157458)](http://a.abcam.com/ps/datasheet/images/157/ab157458/ATP6V1D-Primary-antibodies-ab157458-6.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ATP6V1D antibody [EPR11326(B)] (ab157458)Immunofluorescent analysis of HeLa cells labeling ATP6V1D with ab157458 at 1/100 dilution. -
![Immunoprecipitation - Anti-ATP6V1D antibody [EPR11326(B)] (ab157458)](http://a.abcam.com/ps/datasheet/images/157/ab157458/ATP6V1D-Primary-antibodies-ab157458-7.jpg)
Immunoprecipitation - Anti-ATP6V1D antibody [EPR11326(B)] (ab157458)Anti-ATP6V1D antibody [EPR11326(B)] (ab157458) at 1/10000 dilution + Immunoprecipitation pellet from Y79 cell lysate at 10 µg
Protocols
References
ab157458 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"