Overview

  • Product nameAnti-ATP6V1E2 antibody
    See all ATP6V1E2 primary antibodies
  • Description
    Mouse polyclonal to ATP6V1E2
  • SpecificityThis antibody is reactive against mammalian transfected lysate.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    ATP6V1E2 (NP_542384.1, 1 a.a. ~ 226 a.a) full-length human protein.

  • Positive control
    • ATP6V1E2 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab68011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 26 kDa).

Target

  • FunctionSubunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. This isoform is essential for energy coupling involved in acidification of acrosome.
  • Tissue specificityTestis specific.
  • Sequence similaritiesBelongs to the V-ATPase E subunit family.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP6V1E2 antibody
    • ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E2 antibody
    • V-ATPase subunit E 2 antibody
    • V-type proton ATPase subunit e 2 antibody
    • Vacuolar proton pump subunit e 2 antibody
    • vacuolar type proton-translocating ATPase subunit E1 antibody
    • VATE2_HUMAN antibody
    see all

Anti-ATP6V1E2 antibody images

  • All lanes : Anti-ATP6V1E2 antibody (ab68011) at 1/500 dilution

    Lane 1 : ATP6V1E2 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 26 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)

References for Anti-ATP6V1E2 antibody (ab68011)

ab68011 has not yet been referenced specifically in any publications.

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