Overview

  • Product nameAnti-ATP6V1G3 antibody
    See all ATP6V1G3 primary antibodies
  • Description
    Rabbit polyclonal to ATP6V1G3
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    amino acids 36-115 (AKEEAMVEID QYRMQRDKEF RLKQSKIMGS QNNLSDEIEE QTLGKIQELN GHYNKYMESV MNQLLSMVCD MKPEIHVNYR) of Human ATP6V1G3 (Q96LB4).

  • Positive control
    • RT-4, U-251 MG, Human liver and tonsil lysate. Human kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab122012 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/250 - 1/500.
IHC-P 1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionCatalytic subunit of the peripheral V1 complex of vacuolar ATPase (V-ATPase). V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.
  • Tissue specificityKidney.
  • Sequence similaritiesBelongs to the V-ATPase G subunit family.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP6G3 antibody
    • atp6v1g3 antibody
    • ATPase, H+ transporting, lysosomal (vacuolar proton pump) subunit G3 antibody
    • ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G3 antibody
    • MGC119810 antibody
    • MGC119813 antibody
    • MGC130213 antibody
    • MGC130214 antibody
    • V ATPase 13 kDa subunit 3 antibody
    • V ATPase G subunit 3 antibody
    • V ATPase G3 subunit antibody
    • V ATPase subunit G 3 antibody
    • V-ATPase 13 kDa subunit 3 antibody
    • V-ATPase subunit G 3 antibody
    • V-type proton ATPase subunit G 3 antibody
    • Vacuolar ATP synthase subunit G 3 antibody
    • Vacuolar proton pump G subunit 3 antibody
    • Vacuolar proton pump subunit G 3 antibody
    • Vacuolar proton pump, subunit G3 antibody
    • VATG3_HUMAN antibody
    • Vma10 antibody
    see all

Anti-ATP6V1G3 antibody images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab122012 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-ATP6V1G3 antibody (ab122012) at 1/250 dilution

    Lane 1 : RT-4 lysate
    Lane 2 : U-251 MG lysate
    Lane 3 : Human plasma.
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

    Developed using the ECL technique
  • ab122012, at a 1/2500 dilution, staining ATP6V1G3 in paraffin-embedded Human kidney by Immunohistochemistry.

References for Anti-ATP6V1G3 antibody (ab122012)

ab122012 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"