Overview

  • Product nameAnti-ATP7A antibody
    See all ATP7A primary antibodies
  • Description
    Rabbit polyclonal to ATP7A
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cat, Dog, Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to Human ATP7A. Short peptide sequence used to raise this antibody is 100% homologous to isoform 4 (1500aa, 163kDa), 1 (1514aa, 165kDa), 2 (1581aa, 172kDa), 5 (1422aa, 154kDa) of human ATP7A
    Database link: Q04656

  • Positive control
    • WB: HepG2 cell lysate. IHC-P: Human kidney

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab42486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 30 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionMay supply copper to copper-requiring proteins within the secretory pathway, when localized in the trans-Golgi network. Under conditions of elevated extracellular copper, it relocalized to the plasma membrane where it functions in the efflux of copper from cells.
  • Tissue specificityFound in most tissues except liver. Isoform 3 is widely expressed including in liver cell lines. Isoform 1 is expressed in fibroblasts, choriocarcinoma, colon carcinoma and neuroblastoma cell lines. Isoform 2 is expressed in fibroblasts, colon carcinoma and neuroblastoma cell lines.
  • Involvement in diseaseMenkes disease
    Occipital horn syndrome
    Distal spinal muscular atrophy, X-linked, 3
  • Sequence similaritiesBelongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.
    Contains 6 HMA domains.
  • DomainThe C-terminal di-leucine, 1487-Leu-Leu-1488, is an endocytic targeting signal which functions in retrieving recycling from the plasma membrane to the TGN. Mutation of the di-leucine signal results in the accumulation of the protein in the plasma membrane.
  • Cellular localizationEndoplasmic reticulum; Cytoplasm > cytosol and Golgi apparatus > trans-Golgi network membrane. Cell membrane. Cycles constitutively between the trans-Golgi network (TGN) and the plasma membrane. Predominantly found in the TGN and relocalized to the plasma membrane in response to elevated copper levels.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP7A antibody
    • ATP7A_HUMAN antibody
    • Copper pump 1 antibody
    • Copper-transporting ATPase 1 antibody
    • DSMAX antibody
    • MC1 antibody
    • Menkes disease ATPase antibody
    • Menkes disease-associated protein antibody
    • Menkes syndrome antibody
    • MK antibody
    • MNK antibody
    • OHS antibody
    • SMAX3 antibody
    see all

Anti-ATP7A antibody images

  • Antibody ab42486 (4.0-8.0 ug/ml) immunohistochemistry staining of paraffin embedded human kidney. Positive staining of epithelial cells of human renal tubules idicated by arrows.Antibody ab42486 (4.0-8.0 ug/ml) immunohistochemistry staining of paraffin embedded human kidney. Positive staining of epithelial cells of human renal tubules idicated by arrows.
  • ICC/IF image of ab42486 stained Mcf7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42486, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-ATP7A antibody (ab42486)

This product has been referenced in:
  • Anandhan A  et al. Overexpression of alpha-synuclein at non-toxic levels increases dopaminergic cell death induced by copper exposure via modulation of protein degradation pathways. Neurobiol Dis 81:76-92 (2015). WB ; Rat . Read more (PubMed: 25497688) »

See 1 Publication for this product

Product Wall



We are still currently investigating the issue with ab42486. We are reviewingthe targetof this antibody and the datasheet will beupdated as soonas possible.

We don not have any information about the expression of ATP7A in muscle, ho...

Read More

Thank you for your emails.

I have received your request for the replacement of ab42486with ab131400. Please note that ab131400 is not currently in stock and should be delivered to you within one week, maximum 2.

In order to process ...

Read More

Thank you for your message and for providing this further information.

It looks likewrong information was entered for ab42486. My colleaguesin charge of the product datasheets are reviewing this problem and the websitewill beupdated as soon a...

Read More

Thank you very much for contacting us and for reporting this inconsistency. I just wanted to let you know that I am still working on your inquiry. I have contacted the originator of this product for the information you requested, and I am awaiting a...

Read More

Thank you for your enquiry. I can confirm there are in two different isoforms for this protein, The 30 KD protein is a much shorter alternate isoform that is listed in both the NCBI an SwissProt databases and is sometimes detected for ATP7A. ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"