• Product nameAnti-ATP7b antibody [EPR6794]
    See all ATP7b primary antibodies
  • Description
    Rabbit monoclonal [EPR6794] to ATP7b
  • Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues on the C-terminus of the Human ATP7B protein (P35670).

  • Positive control
    • Caco-2 and HepG2 cell lysates and HepG2 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab124973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 157 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for IHC-P or IP.
  • Target

    • FunctionInvolved in the export of copper out of the cells, such as the efflux of hepatic copper into the bile.
    • Tissue specificityMost abundant in liver and kidney and also found in brain. Isoform 2 is expressed in brain but not in liver. The cleaved form WND/140 kDa is found in liver cell lines and other tissues.
    • Involvement in diseaseDefects in ATP7B are the cause of Wilson disease (WD) [MIM:277900]. WD is an autosomal recessive disorder of copper metabolism in which copper cannot be incorporated into ceruloplasmin in liver, and cannot be excreted from the liver into the bile. Copper accumulates in the liver and subsequently in the brain and kidney. The disease is characterized by neurologic manifestations and signs of cirrhosis.
    • Sequence similaritiesBelongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.
      Contains 6 HMA domains.
    • Post-translational
      Isoform 1 may be proteolytically cleaved at the N-terminus to produce the WND/140 kDa form.
    • Cellular localizationCytoplasm; Mitochondrion and Golgi apparatus > trans-Golgi network membrane. Predominantly found in the trans-Golgi network (TGN). Not redistributed to the plasma membrane in response to elevated copper levels.
    • Information by UniProt
    • Database links
    • Alternative names
      • ATP7B antibody
      • ATP7B_HUMAN antibody
      • ATPase, Cu(2+) transporting, beta polypeptide antibody
      • ATPase, Cu++ transporting, beta polypeptide antibody
      • Copper pump 2 antibody
      • Copper transporting ATPase 2 antibody
      • PWD antibody
      • Toxic milk antibody
      • tx antibody
      • WC1 antibody
      • WD antibody
      • Wilson disease associated protein antibody
      • Wilson disease-associated protein antibody
      • WND antibody
      • WND/140 kDa antibody
      see all

    Anti-ATP7b antibody [EPR6794] images

    • All lanes : Anti-ATP7b antibody [EPR6794] (ab124973) at 1/1000 dilution

      Lane 1 : Caco-2 cell lysate
      Lane 2 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 157 kDa
    • Flow cytometric analysis of permeabilized HepG2 cells using anti-ATP7B RabMAb in red (ab124973) or a rabbit IgG in green(negative).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-ATP7b antibody [EPR6794] (ab124973)

    This product has been referenced in:

    See 1 Publication for this product

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