Overview
- Product nameAnti-atpF antibody
- DescriptionRabbit polyclonal to atpF
- Specificityab65382 is specific for AtpF subunit of ATP synthase.
- Tested applicationsWB more details
- Species reactivityReacts with: Arabidopsis thaliana, Plants, Chlamydomonas reinhardtii
- Immunogen
Full length protein: atpF subunit of the chloroplast ATP synthase complex extracted from Arabidopsis thaliana.
- Positive controlArabidopsis and spinach chloroplast proteins.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.01% Sodium Azide
- PurityWhole antiserum
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab65382 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/5000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). |
Target
- RelevanceatpF belongs to the family of F1-type ATPases, which are also present in bacteria and mitochondria. ATP synthase generates ATP from ADP and inorganic phosphate using energy derived from a trans-thylakoidal electrochemical proton gradient. The transmembrane atpF subunit belongs to a stator part of ATP synthase.
- Cellular localizationPlastid, chloroplast thylakoid membrane; Single-pass membrane protein.
-
Database links
- Entrez Gene: 844789 Arabidopsis thaliana
- Entrez Gene: 2716983 Chlamydomonas reinhardtii
- SwissProt: P56759 Arabidopsis thaliana
- SwissProt: Q8HTL5 Chlamydomonas reinhardtii
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Alternative names
- ATP synthase CF0 B subunit antibodyATPase subunit I antibodyatpF antibodyCF0 ATP synthase subunit I antibody
Anti-atpF antibody images
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All lanes : Anti-atpF antibody (ab65382) at 1/5000 dilution
Lane 1 : Arabidopsis chloroplast proteins
Lane 2 : spinach chloroplast proteins
Lysates/proteins at 5 µg per lane.
developed using the ECL technique
Predicted band size : 21 kDa
Observed band size : 21 kDa
References for Anti-atpF antibody (ab65382)
ab65382 has not yet been referenced specifically in any publications.
