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Western blot - atpF antibody (ab65382)
(enlarge)
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Product Name
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atpF antibody
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to atpF
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Immunogen
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Full length protein: atpF subunit of the chloroplast ATP synthase complex extracted from Arabidopsis thaliana.
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Reacts with
(species key)
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At, C rei, Plnts
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Specificity
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ab65382 is specific for AtpF subunit of ATP synthase.
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/5000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Arabidopsis and spinach chloroplast proteins.
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Cellular localization
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Plastid, chloroplast thylakoid membrane; Single-pass membrane protein.
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Research areas
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Cell Biology >> Other Antibodies >> Plant related targets
Signal Transduction >> Metabolism >> Energy Metabolism
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Relevance
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atpF belongs to the family of F1-type ATPases, which are also present in bacteria and mitochondria. ATP synthase generates ATP from ADP and inorganic phosphate using energy derived from a trans-thylakoidal electrochemical proton gradient. The transmembrane atpF subunit belongs to a stator part of ATP synthase.
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Database links
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The links below go to external sites and will open in a new browser window Entrez Gene | | 844789 (Arabidopsis thaliana)
2716983 (Chlamydomonas reinhardtii)
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| SwissProt | | P56759 (Arabidopsis thaliana)
Q8HTL5 (Chlamydomonas reinhardtii)
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: 0.01% Sodium Azide
Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this atpF antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for atpF antibody images, references, products related to ab65382 and other tools.
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atpF antibody images:
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 Western blot - atpF antibody (ab65382)
All lanes : atpF antibody (ab65382) at 1/5000 dilution
Lane 1 : Arabidopsis chloroplast
proteins
Lane 2 : spinach chloroplast
proteins
Lysates/proteins at 5 µg per lane.
developed using the ECL technique
Predicted band size : 21 kDa
Observed band size : 21 kDa
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Search PubMed (MEDLINE) for references to atpF
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atpF antibody - more information
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Customer reviews (feedback) regarding atpF antibody |
Customer FAQs regarding atpF antibody |
Protocols for atpF antibody |
Price and availability of products related to atpF antibody |
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atpF antibody - related products:
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atpF antibody - other tools:
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Contact Abcam with a Technical Enquiry about atpF antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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