Overview

  • Product name
    Anti-ATPase Inhibitory Factor 1 antibody
    See all ATPase Inhibitory Factor 1 primary antibodies
  • Description
    Rabbit polyclonal to ATPase Inhibitory Factor 1
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide (Human)

  • Positive control
    • 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab81061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 12 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent dilution.
ICC/IF Use at an assay dependent concentration. PubMed: 22684333

Target

  • Function
    Endogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix.
  • Sequence similarities
    Belongs to the ATPase inhibitor family.
  • Post-translational
    modifications
    Exhibits variability in chain length, mitochondria have distinct pools of protein cleaved after the 24th, 25th, and 26th amino acid.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATIF1_HUMAN antibody
    • ATP synthase inhibitor protein antibody
    • ATPase inhibitor antibody
    • ATPase inhibitor mitochondrial antibody
    • ATPase inhibitor protein antibody
    • ATPase inhibitory factor 1 antibody
    • ATPI antibody
    • ATPIF 1 antibody
    • Atpif1 antibody
    • ATPIP antibody
    • IF(1) antibody
    • IF1 antibody
    • Inhibitor of F(1)F(o)-ATPase antibody
    • IP antibody
    • MGC1167 antibody
    • MGC8898 antibody
    • mitochondrial antibody
    see all

Images

  • Anti-ATPase Inhibitory Factor 1 antibody (ab81061) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 12 kDa
    Observed band size : 10 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Capparelli C  et al. CTGF drives autophagy, glycolysis and senescence in cancer-associated fibroblasts via HIF1 activation, metabolically promoting tumor growth. Cell Cycle 11:2272-84 (2012). ICC/IF ; Human . Read more (PubMed: 22684333) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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