The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 12 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent dilution.
Use at an assay dependent concentration. PubMed: 22684333
FunctionEndogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix.
Sequence similaritiesBelongs to the ATPase inhibitor family.
Post-translational modificationsExhibits variability in chain length, mitochondria have distinct pools of protein cleaved after the 24th, 25th, and 26th amino acid.
References for Anti-ATPase Inhibitory Factor 1 antibody (ab81061)
This product has been referenced in:
Capparelli C et al. CTGF drives autophagy, glycolysis and senescence in cancer-associated fibroblasts via HIF1 activation, metabolically promoting tumor growth. Cell Cycle11:2272-84 (2012).
Read more (PubMed: 22684333) »