Anti-ATPase Na+/ K+ beta 2 antibody (ab76509)

Overview

  • Product nameAnti-ATPase Na+/ K+ beta 2 antibody
    See all ATPase Na+/ K+ beta 2 primary antibodies
  • Description
    Mouse monoclonal to ATPase Na+/ K+ beta 2
  • Specificityreacts with recombinant fragment used as immunogen
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Human
  • Immunogen

    Recombinant fragment: IRPKTENLDV IVNVSDTESW DQHVQKLNKF LEPYNDSIQA QKNDVCRPGR YYEQPDNGVL NYPKRACQFN RTQLGNCSGI GDSTHYGYST GQPCVFIKMN RVINFYAGAN , corresponding to amino acids 84-194 of Human ATPase Na+/ K+ beta 2, with tag (NP_001669)

  • Positive control
    • immunogen

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: Use at an assay dependent dilution.
    sELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 32 kDa.
    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionThis is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-2 subunit is not known.
    • Sequence similaritiesBelongs to the X(+)/potassium ATPases subunit beta family.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • adhesion molecule on glia antibody
      • AMOG antibody
      • AT1B2 antibody
      • AT1B2_HUMAN antibody
      • ATP1B2 antibody
      • ATPase Na+/K+ transporting beta 2 polypeptide antibody
      • ATPB2 antibody
      • ATPB2S antibody
      • MGC93648 antibody
      • Na+/K+ -ATPase beta 2 subunit antibody
      • Na, K ATPase beta 2 polypeptide antibody
      • RATATPB2S antibody
      • sodium potassium ATPase subunit beta 2 (non-catalytic) antibody
      • sodium pump subunit beta 2 antibody
      • sodium/potassium dependent ATPase beta 2 subunit antibody
      • Sodium/potassium dependent ATPase subunit beta 2 antibody
      • sodium/potassium transporting ATPase beta 2 chain antibody
      • sodium/potassium transporting ATPase subunit beta 2 antibody
      • Sodium/potassium-dependent ATPase subunit beta-2 antibody
      • Sodium/potassium-transporting ATPase subunit beta-2 antibody
      see all

    Anti-ATPase Na+/ K+ beta 2 antibody images

    • Anti-ATPase Na+/ K+ beta 2 antibody (ab76509) at 1/250 dilution + Mouse 3T3 whole cell lysate at 25 µg

      Secondary
      HRP-conjugated Goat anti-mouse IgG polyclonal at 1/2000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 32 kDa
      Observed band size : 32 kDa
      Additional bands at : 45 kDa (possible non-specific binding).

      Exposure time : 1 minute

      This image is courtesy of an anonymous Abreview

      See Abreview

    • Anti-ATPase Na+/ K+ beta 2 antibody (ab76509) at 5 µg/ml + immunogen at 0.2 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

      Predicted band size : 32 kDa
      Observed band size : 38 kDa (why is the actual band size different from the predicted?)

    References for Anti-ATPase Na+/ K+ beta 2 antibody (ab76509)

    This product has been referenced in:
    • Xu R  et al. Oxidative stress mediates the disruption of airway epithelial tight junctions through a TRPM2-PLC?1-PKCa signaling pathway. Int J Mol Sci 14:9475-86 (2013). WB ; Human . Read more (PubMed: 23629676) »
    • McIntyre A  et al. Carbonic anhydrase IX promotes tumor growth and necrosis in vivo and inhibition enhances anti-VEGF therapy. Clin Cancer Res 18:3100-11 (2012). WB . Read more (PubMed: 22498007) »

    See all 2 Publications for this product

    Product Wall

    Application Western blot
    Sample Mouse Tissue lysate - whole (Heart)
    Gel Running Conditions Reduced Denaturing (4-15% acrylamide gel, SDS)
    Loading amount 30 µg
    Specification Heart
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Dr. Pierre Thiebaut

    Verified customer

    Submitted Aug 19 2015

    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (J774A.1 macrophages)
    Permeabilization Yes - 0.05% Saponin
    Specification J774A.1 macrophages
    Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
    Fixative Methanol
    Username

    Abcam user community

    Verified customer

    Submitted Jul 14 2015

    Application Western blot
    Loading amount 25 µg
    Gel Running Conditions Reduced Denaturing (12%)
    Sample Mouse Cell lysate - whole cell (3T3)
    Specification 3T3
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 25 2013

    Thank you for contacting us.

    Yes, the antibody ab7671 will detect the protein in neurons.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use o...

    Read More

    Thank you for contacting us.

    The antibody we have are ab7671; http://www.abcam.com/alpha-1-Sodium-Potassium-ATPase-antibody-464-6-Plasma-Membrane-Marker-ab7671.html;

    The immunogen sequence of ab76509 is quite similar with rat seque...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"