Anti-ATPG antibody [2A1AA11] (ab119686)

  Western blot - Anti-ATPG antibody [2A1AA11] (ab119686)

All lanes : Anti-ATPG antibody [2A1AA11] (ab119686) at 1 µg/ml

Lane 1 : human heart homogenate lysate at 15 µg
Lane 2 : human HepG2 cell lysate at 15 µg
Lane 3 : human liver mitochondria lysate at 7.5 µg
Lane 4 : rat liver mitochondria lysate at 7.5 µg
Lane 5 : mouse liver mitochondria lysate at 7.5 µg
Lane 6 : bovine heart mitochondria lysate at 7.5 µg

Secondary
Goat anti-mouse HRP at 1/5000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 33 kDa

  Immunocytochemistry/ Immunofluorescence - Anti-ATPG antibody [2A1AA11] (ab119686)

Immunocytochemistry using ab119686 stained HDFn cells (human). The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min) with antigen retrieval. The cells were then incubated with the antibody (ab119686, 1µg/ml) for 2h at room temperature or over night at 4°C. The secondary antibody was (red) 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondria.

  Flow Cytometry - Anti-ATPG antibody [2A1AA11] (ab119686)

Flow cytometry. Hela cells were stained with 1µg/mL anti-ATPG antibody (ab119686) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.