Overview

  • Product name
  • Description
    Rabbit polyclonal to ATR
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human ATR.

  • Positive control
    • This antibody gave a positive signal mouse and rat testis tissue lysates and in the following whole cell lysates: HeLa; U20S; K562; Raji.

Properties

Applications

Our Abpromise guarantee covers the use of ab101900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 250 kDa (predicted molecular weight: 301 kDa).

Target

  • Function
    Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication.
  • Tissue specificity
    Ubiquitous, with highest expression in testis. Isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary.
  • Involvement in disease
    Defects in ATR are a cause of Seckel syndrome type 1 (SCKL1) [MIM:210600]. SCKL1 is a rare autosomal recessive disorder characterized by growth retardation, microcephaly with mental retardation, and a characteristic 'bird-headed' facial appearance.
  • Sequence similarities
    Belongs to the PI3/PI4-kinase family. ATM subfamily.
    Contains 1 FAT domain.
    Contains 1 FATC domain.
    Contains 2 HEAT repeats.
    Contains 1 PI3K/PI4K domain.
  • Post-translational
    modifications
    Phosphorylated; autophosphorylates in vitro.
  • Cellular localization
    Nucleus. Nucleus > PML body. Depending on the cell type, it can also be found in PML nuclear bodies. Recruited to chromatin during S-phase. Redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ataxia telangiectasia and Rad3 related antibody
    • Ataxia telangiectasia and Rad3-related protein antibody
    • ATR antibody
    • ATR_HUMAN antibody
    • FCTCS antibody
    • FRAP Related Protein 1 antibody
    • FRAP-related protein 1 antibody
    • FRP1 antibody
    • MEC1 antibody
    • MEC1 mitosis entry checkpoint 1 homolog antibody
    • Protein kinase ATR antibody
    • RAC3 antibody
    • Rad3 related protein antibody
    • SCKL antibody
    • SCKL1 antibody
    • Serine/threonine protein kinase ATR antibody
    • Serine/threonine-protein kinase ATR antibody
    see all

Images

  • All lanes : Anti-ATR antibody (ab101900) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
    Lane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
    Lane 4 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with immunising peptide at 1 µg/ml
    Lane 6 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate with immunising peptide at 1 µg/ml
    Lane 7 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate with immunising peptide at 1 µg/ml
    Lane 8 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate with immunising peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 301 kDa
    Observed band size : 250 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 41 kDa,67 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • All lanes : Anti-ATR antibody (ab101900) at 1 µg/ml

    Lane 1 : Testis (Mouse) Tissue Lysate
    Lane 2 : Testis (Rat) Tissue Lysate - normal tissue (ab29388)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 301 kDa
    Observed band size : 250 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa,70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

References

ab101900 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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