Overview

  • Product nameAnti-ATR antibodySee all ATR primary antibodies ...
  • Description
    Rabbit polyclonal to ATR
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Mouse, Hamster, Cow, Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 2122-2380 of Human ATR.

  • General notesThis product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab10944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/2000. Predicted molecular weight: 317.3 kDa.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionSerine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication.
    • Tissue specificityUbiquitous, with highest expression in testis. Isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary.
    • Involvement in diseaseDefects in ATR are a cause of Seckel syndrome type 1 (SCKL1) [MIM:210600]. SCKL1 is a rare autosomal recessive disorder characterized by growth retardation, microcephaly with mental retardation, and a characteristic 'bird-headed' facial appearance.
    • Sequence similaritiesBelongs to the PI3/PI4-kinase family. ATM subfamily.
      Contains 1 FAT domain.
      Contains 1 FATC domain.
      Contains 2 HEAT repeats.
      Contains 1 PI3K/PI4K domain.
    • Post-translational
      modifications
      Phosphorylated; autophosphorylates in vitro.
    • Cellular localizationNucleus. Nucleus > PML body. Depending on the cell type, it can also be found in PML nuclear bodies. Recruited to chromatin during S-phase. Redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling.
    • Target information above from: UniProt accession Q13535 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • ACTR antibody
      • AIB1 antibody
      • amplified in breast cancer 1 antibody
      • Ataxia Telangiecasia and Rad3 Related antibody
      • Ataxia telangiectasia and Rad3 related antibody
      • Ataxia telangiectasia and Rad3-related protein antibody
      • ATR antibody
      • ATR_HUMAN antibody
      • CAGH16 antibody
      • CBP interacting protein antibody
      • CTG26 antibody
      • FCTCS antibody
      • FRAP Related Protein 1 antibody
      • FRAP-related protein 1 antibody
      • FRP 1 antibody
      • FRP1 antibody
      • Human JTV 1 antibody
      • MEC1 antibody
      • MEC1 mitosis entry checkpoint 1 homolog antibody
      • nuclear receptor coactivator 3 antibody
      • pCIP antibody
      • protein kinase ATR antibody
      • RAC3 antibody
      • Rad3 related protein antibody
      • receptor associated coactivator 3 antibody
      • SCKL antibody
      • SCKL1 antibody
      • Seckel syndrome antibody
      • Serine/threonine protein kinase ATR antibody
      • Serine/threonine-protein kinase ATR antibody
      • steroid receptor coactivator protein 3 antibody
      • thyroid hormone receptor activator molecule 1 antibody
      • TRAM1 antibody
      see all

    References for Anti-ATR antibody (ab10944)

    ab10944 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Human Cell lysate - whole cell (HCT116p53wt cells)
    Loading amount 40 µg
    Specification HCT116p53wt cells
    Gel Running Conditions Reduced Denaturing (4-20% Tris-Gly gel)
    Blocking step LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
    Username

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    Submitted Nov 17 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"