Overview

  • Product nameAnti-ATR antibody
    See all ATR primary antibodies
  • Description
    Mouse monoclonal to ATR
  • Tested applicationsSuitable for: ICC/IF, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey
  • Immunogen

    Recombinant fragment: DQREPLMSVL KTFLHDPLVE WSKPVKGHSK APLNETGEVV NEKAKTHVLD IEQRLQGVIK TRNRVTGLPL SIEGHVHYLI QEATDENLLC QMYLGWTPYM , corresponding to amino acids 2545-2645 of Human ATR (NP_001175) with tag.

  • Positive control
    • Recombinant tagged ATR protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab54793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged ATR is approximately 0.3ng/ml if used as a capture antibody.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 301 kDa.

Target

  • FunctionSerine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication.
  • Tissue specificityUbiquitous, with highest expression in testis. Isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary.
  • Involvement in diseaseDefects in ATR are a cause of Seckel syndrome type 1 (SCKL1) [MIM:210600]. SCKL1 is a rare autosomal recessive disorder characterized by growth retardation, microcephaly with mental retardation, and a characteristic 'bird-headed' facial appearance.
  • Sequence similaritiesBelongs to the PI3/PI4-kinase family. ATM subfamily.
    Contains 1 FAT domain.
    Contains 1 FATC domain.
    Contains 2 HEAT repeats.
    Contains 1 PI3K/PI4K domain.
  • Post-translational
    modifications
    Phosphorylated; autophosphorylates in vitro.
  • Cellular localizationNucleus. Nucleus > PML body. Depending on the cell type, it can also be found in PML nuclear bodies. Recruited to chromatin during S-phase. Redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ataxia telangiectasia and Rad3 related antibody
    • Ataxia telangiectasia and Rad3-related protein antibody
    • ATR antibody
    • ATR_HUMAN antibody
    • FCTCS antibody
    • FRAP Related Protein 1 antibody
    • FRAP-related protein 1 antibody
    • FRP1 antibody
    • MEC1 antibody
    • MEC1 mitosis entry checkpoint 1 homolog antibody
    • Protein kinase ATR antibody
    • RAC3 antibody
    • Rad3 related protein antibody
    • SCKL antibody
    • SCKL1 antibody
    • Serine/threonine protein kinase ATR antibody
    • Serine/threonine-protein kinase ATR antibody
    see all

Anti-ATR antibody images

  • Anti-ATR antibody (ab54793) at 1 µg/ml + Recombinant tagged ATR fragment at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

    Predicted band size : 301 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • ab54793 at 10 ug/ml staining ATR in human Hela cells by Immunocytochemistry / Immunofluorescence.

References for Anti-ATR antibody (ab54793)

ab54793 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hela cells)
Loading amount 50 µg
Specification Hela cells
Treatment UV irradiation at 20J/m2
Gel Running Conditions Reduced Denaturing (4-15% gradient Tris Glycine)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Dec 10 2009

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