Overview

  • Product nameAnti-ATR antibody - ChIP Grade
    See all ATR primary antibodies
  • Description
    Rabbit polyclonal to ATR - ChIP Grade
  • Tested applicationsSuitable for: ICC/IF, IP, WB, ChIPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Fusion protein, corresponding to amino acids 400-460 of Human ATR.

  • Positive control
    • UV irradiated K562 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab2905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
IP Use a concentration of 2 mg/ml.
WB 1/1000. Detects a band of approximately 305 kDa (predicted molecular weight: 309 kDa). (in lysate from UV irradiated K562 cells).
ChIP Use at an assay dependent dilution. PubMed: 20647539

Target

  • FunctionSerine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication.
  • Tissue specificityUbiquitous, with highest expression in testis. Isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary.
  • Involvement in diseaseDefects in ATR are a cause of Seckel syndrome type 1 (SCKL1) [MIM:210600]. SCKL1 is a rare autosomal recessive disorder characterized by growth retardation, microcephaly with mental retardation, and a characteristic 'bird-headed' facial appearance.
  • Sequence similaritiesBelongs to the PI3/PI4-kinase family. ATM subfamily.
    Contains 1 FAT domain.
    Contains 1 FATC domain.
    Contains 2 HEAT repeats.
    Contains 1 PI3K/PI4K domain.
  • Post-translational
    modifications
    Phosphorylated; autophosphorylates in vitro.
  • Cellular localizationNucleus. Nucleus > PML body. Depending on the cell type, it can also be found in PML nuclear bodies. Recruited to chromatin during S-phase. Redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ataxia telangiectasia and Rad3 related antibody
    • Ataxia telangiectasia and Rad3-related protein antibody
    • ATR antibody
    • ATR_HUMAN antibody
    • FCTCS antibody
    • FRAP Related Protein 1 antibody
    • FRAP-related protein 1 antibody
    • FRP1 antibody
    • MEC1 antibody
    • MEC1 mitosis entry checkpoint 1 homolog antibody
    • Protein kinase ATR antibody
    • RAC3 antibody
    • Rad3 related protein antibody
    • SCKL antibody
    • SCKL1 antibody
    • Serine/threonine protein kinase ATR antibody
    • Serine/threonine-protein kinase ATR antibody
    see all

Anti-ATR antibody - ChIP Grade images



  • Predicted band size : 309 kDa
    Western blot on ATR in K562 whole cell lysate using ab2905.
  • All lanes : Anti-ATR antibody - ChIP Grade (ab2905) at 1/1000 dilution

    Lane 1 : MCF10A breast epithelial cells, grown in normoxic conditions (20% O2). Cells were lysed with NP40.
    Lane 2 : MCF10A Breast Epithelial cells grown in hypoxic. conditions (5% O2). Cells were lysed with NP40.

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat anti Rabbit (HRP) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 309 kDa
    Observed band size : 300 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

    Blocking: Carried out using non fat dry milk for 1 hr at room temperature.

    This image is an edited version of an image submitted courtesy of an Abreview by Francisco Ramirez-Valle submitted on 15th November 2005. We do not have any further information relating to this image.

    See Abreview

  • ab2905 staining ATR in the U2OS cell line from Human Osteosarcoma by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with Formaldehyde, permeabilized with NP-40 and blocked with 3% BSA for 60 minutes at 21°C. Samples were incubated with primary antibody (1/500 in PBS + 3% BSA) for 12 hour at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit IgG polyclonal(1/500) was used as the secondary antibody.

    See Abreview

References for Anti-ATR antibody - ChIP Grade (ab2905)

This product has been referenced in:
  • Huang R  et al. The role of HDAC2 in chromatin remodelling and response to chemotherapy in ovarian cancer. Oncotarget 7:4695-711 (2016). WB ; Human . Read more (PubMed: 26683361) »
  • Tsang SH  et al. Host DNA damage response factors localize to merkel cell polyomavirus DNA replication sites to support efficient viral DNA replication. J Virol 88:3285-97 (2014). WB . Read more (PubMed: 24390338) »

See all 10 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Colon)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization No
Specification Colon
Blocking step hydrogen peroxide as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: 24°C
Fixative Formaldehyde
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Dr. Vinicius Kannen Cardoso

Verified customer

Submitted Sep 15 2016

Application Western blot
Sample Human Cell lysate - whole cell (EC CELLS)
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Loading amount 60 µg
Specification EC CELLS
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
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Submitted Apr 22 2016

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 2% · Temperature: RT°C
Sample Human Cell (HeLa cells)
Specification HeLa cells
Permeabilization Yes - 0.2% Triton-X100
Fixative Paraformaldehyde
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Submitted Jun 20 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Sample Human Cell (Colon cancer)
Specification Colon cancer
Permeabilization Yes - TRITON 0,1%
Fixative Formaldehyde
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Submitted Apr 14 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (3-8% gradient Tris-acetate gel)
Sample Human Cell lysate - whole cell (TIG-1 primary fibroblasts)
Specification TIG-1 primary fibroblasts
Blocking step LI-COR­ Odyssey­ Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
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Submitted Jan 16 2014

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (3-8%)
Sample Human Cell lysate - whole cell (HCT116)
Specification HCT116
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Dec 18 2013

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing (3-8%)
Sample Chicken Cell lysate - whole cell (DT40)
Specification DT40
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Dec 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (6%)
Sample Human Cell lysate - whole cell (HeLa)
Specification HeLa
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Jul 02 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (U2OS)
Specification U2OS
Fixative Formaldehyde
Permeabilization Yes - NP40
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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Submitted Jan 31 2013

That is really up to you, and what you want to do with the membrane: PVDF membranes tend to show a higher background, however, PVDF tends to be slightly more robust during handling and is easier to strip, whereas NC binds the protein stronger, but is m...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"