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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ATR aa 400-500 (phospho S428) (Cysteine residue). The exact sequence is proprietary.
Database link: Q13535
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab178407 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 300 kDa.|
Blocking/Diluting buffer 5% NFDM/TBST
Immunohistochemical analysis of paraffin embedded Human prostate tissue labeling ATR (phospho S428) with ab178407 at 1/50.
Immunohistochemical analysis of paraffin embedded Human testis tissue labeling ATR (phospho S428) with ab178407 at 1/50.
Dot Blot analysis of Lane 1: ATR (pS428) phospho peptide and Lane 2: ATR non-phospho peptide labeling ATR (phospho S428) with ab178407 at 1/1000 dilution. 5% NFDM/TBST was used as the diluting and blocking buffer. ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 10 seconds.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"