Overview

  • Product name
    Anti-AtRabA4 antibody
  • Description
    Rabbit polyclonal to AtRabA4
  • Specificity
    ab55896 specifically recognizes recombinant AtRabA4b fusion protein
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Arabidopsis thaliana, Soybean
  • Immunogen

    Synthetic peptide corresponding to Arabidopsis thaliana AtRabA4 aa 201-219 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    GKKILIPGSGQEIPAKTST


    Database link: Q38923

  • Positive control
    • Whole cell extract of E. coli expressing AtRabA4b fusion protein
  • General notes
    If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Properties

Applications

Our Abpromise guarantee covers the use of ab55896 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 24 kDa. Staining of the AtRabA4b band in immunoblotting is specifically inhibited by the immunizing peptide.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Rab GTPases belong to the superfamily of small GTP binding proteins that include Ras, Rho, Arf and Ran GTPases. The Rab GTPases are involved in the regulation of diverse eukaryotic cellular processes such as cytoskeletal assembly and organization, cellproliferation and intracellular membrane trafficking. In eukaryotic cells Rab GTPases regulate membrane trafficking events associated with distinct compartments. The type of subcellular compartment with which Rab GTPase is associated can be predicted based on sequence similarity. The Arabidopsis genome contains a total of 93 small GTPase genes, including 57 members of the Rab family. Arabidopsis thaliana Rab GTPase RabA4b is highly similar to AtRab11. AtRabA4b labels a novel compartment that accumulates at the tips of expanding root hair cells. It has been suggested that in A. thaliana, the RabA4b GTPase regulates membrane trafficking steps involved in the polarized deposition of cell wall components in tip-growing root hair cells. AtRabA4b has been shown to label a novel, trans-Golgi network compartment displaying a developmentally regulated polar distribution in growing A. thaliana root hair cells. GTP bound AtRabA4b selectively recruits the plant phosphatidylinositol-4- kinase PI-4Kb1, but not other members of PI-4K families.
    • Database links

      References

      ab55896 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      There are currently no Customer reviews or Questions for ab55896.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

      Sign up