Overview

  • Product name
    Anti-AtRAC3 antibody
  • Description
    Rabbit polyclonal to AtRAC3
  • Specificity
    Sequence used for immunogen is identical in AtRac 1, 2, 4, 5, 6, 8, 10 and 11 and in RAC/Rop of many plant species. It is highly conserved (single amino acid substitution)in AtRac7 and AtRac9
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Arabidopsis thaliana
  • Immunogen

    Synthetic peptide:

    TAGQEDYNRLRPLSYRGAD

    conjugated to KLH, corresponding to N terminal amino acids 61-79 of Arabidopsis thaliana RAC3

Properties

Applications

Our Abpromise guarantee covers the use of ab50678 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).

Target

  • Relevance
    Plants contain a unique Rho GTPase subfamily designated as RAC or Rop (Rho from plants), subdivided into two major subgroups according to whether they are prenylated or palmitoylated. RAC/Rop proteins regulate a wide array of cellular processes. They induce reorganization of actin, and have been implicated in changes in polar growth patterns, inducing Ca2+ influx at the tip in germinating pollen tubes, regulating various hormone-mediated plant signalling and developmental processes, the production of reactive oxygen species and modulating the pathogen-induced hypersensitive response (HR). In Arabidopsis, 11 different RAC/Rop GTPases have been identified so far, but the function of the individual RACs is not yet fully understood.
  • Cellular localization
    Cytoplasmic and Cell Membrane; peripheral membrane protein. Note: Associated with the membrane when activated.
  • Database links
    • Alternative names
      • ARAC3 antibody
      • ATROP6 antibody
      • GTPase protein ROP6 antibody
      • Rac like GTP binding protein ARAC3 antibody
      • Rho related protein from plants 6 antibody
      • RHO1PS antibody
      • ROP6 antibody
      see all

    Images

    • All lanes : Anti-AtRAC3 antibody (ab50678) at 1/2000 dilution

      Lane 1 : E. coli extract expressing AtRAC3 (10µg)
      Lane 2 : E. coli extract expressing AtRAC3 (5µg)
      Lane 3 : E. coli extract expressing AtRAC3 (2.5µg)
      Lane 4 : E. coli extract expressing AtRAC3 (1.2µg)
      Lane 5 : E. coli extract expressing AtRAC3 (0.6µg)
      Lane 6 : E. coli extract expressing AtRAC3 (5mg) with immunizing peptide at 10 µg/ml

      Secondary
      Goat Anti-Rabbit IgG, Peroxidase conjugate
      Developed using the ECL technique

      Predicted band size : 22 kDa
      Observed band size : 22 kDa

    References

    ab50678 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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