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Our Abpromise guarantee covers the use of ab17962 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/15000. Detects a band of approximately 95 kDa (predicted molecular weight: 86.8 kDa).|
Detection of Human ATRIP using ab17962 by Western Blot (2 x left images) and Immunoprecipitation (right image).
Samples: Whole cell lysate (100
Antibodies: Affinity purified rabbit anti-ATRIP antibody (ab17962) used at 1/500 and 1/15000 dilution for WB and 3
ATRIP was also immunoprecipitated using a control antibody generated to recombinant human ATRIP:
Right image - lane 1 ab17962; lane 2 control antibody; lane 3 normal IgG.
Immunoprecipitated ATRIP was detected using the antibody to the recombinant protein. Detection: Chemiluminescence with exposure times of 5 minutes (2 x left) and 45 seconds (right image).
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