The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-P: 1/100 - 1/500. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining.
Likely to work with frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionRequired for checkpoint signaling after DNA damage. Required for ATR expression, possibly by stabilizing the protein.
Sequence similaritiesBelongs to the ATRIP family.
DomainThe EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage.
Post-translational modificationsPhosphorylated by ATR.
Cellular localizationNucleus. Redistributes to discrete nuclear foci upon DNA damage.