Overview

  • Product nameAnti-Atrophin 1 antibody
    See all Atrophin 1 primary antibodies
  • Description
    Rabbit polyclonal to Atrophin 1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1150 to the C-terminus of Human Atrophin 1.

    (Peptide available as ab32861.)

  • Positive control
    • This antibody gave a positive signal in Human Testis Tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab32604 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 125 kDa).

Target

Anti-Atrophin 1 antibody images

  • IHC image of ab32604 staining in Human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32604, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Anti-Atrophin 1 antibody (ab32604) at 1 µg/ml + Human testis tissue lysate - total protein (ab30257) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size : 125 kDa
    Observed band size : 125 kDa
    Additional bands at : 100 kDa,55 kDa,76 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Atrophin 1 antibody (ab32604)

ab32604 has not yet been referenced specifically in any publications.

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