Overview

  • Product nameAnti-ATTY antibody
    See all ATTY primary antibodies
  • Description
    Rabbit polyclonal to ATTY
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human ATTY.

    (Peptide available as ab109070.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: NIH3T3; HepG2; PC12; A20; Caco2; SKNBE; MCF7; K562; Raji; A431.

Properties

Applications

Our Abpromise guarantee covers the use of ab91450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).

Target

  • FunctionTransaminase involved in tyrosine breakdown. Converts tyrosine to p-hydroxyphenylpyruvate. Can catalyze the reverse reaction, using glutamic acid, with 2-oxoglutarate as cosubstrate (in vitro). Has no transaminase activity towards phenylalanine.
  • PathwayAmino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 2/6.
  • Involvement in diseaseDefects in TAT are the cause of tyrosinemia type 2 (TYRO2) [MIM:276600]; also known as Richner-Hanhart syndrome. TYRO2 is an inborn error of metabolism characterized by elevations of tyrosine in the blood and urine, and oculocutaneous manifestations. Typical features include palmoplantar keratosis, painful corneal ulcers, and mental retardation.
  • Sequence similaritiesBelongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATTY_HUMAN antibody
    • L-tyrosine:2-oxoglutarate aminotransferase antibody
    • TAT antibody
    • Tyrosine aminotransferase antibody
    • Tyrosine aminotransferase, cytosolic antibody
    see all

Anti-ATTY antibody images

  • All lanes : Anti-ATTY antibody (ab91450) at 1 µg/ml

    Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 4 : A20 whole cell lysate (ab7180)
    Lane 5 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 6 : SK N BE (Human neuroblastoma) Whole Cell Lysate
    Lane 7 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 8 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
    Lane 9 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 10 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 50 kDa
    Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 16 minutes
  • ICC/IF image of ab91450 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab91450, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-ATTY antibody (ab91450)

ab91450 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"