Overview

  • Product nameAnti-ATTY antibody [EPR6121]
    See all ATTY primary antibodies
  • Description
    Rabbit monoclonal [EPR6121] to ATTY
  • Tested applicationsSuitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human ATTY.

  • Positive control
    • HepG2 (treated with insulin), HepG2, and A549 cell lysates; Human brain tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab125000 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
ICC 1/50 - 1/100.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionTransaminase involved in tyrosine breakdown. Converts tyrosine to p-hydroxyphenylpyruvate. Can catalyze the reverse reaction, using glutamic acid, with 2-oxoglutarate as cosubstrate (in vitro). Has no transaminase activity towards phenylalanine.
    • PathwayAmino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 2/6.
    • Involvement in diseaseDefects in TAT are the cause of tyrosinemia type 2 (TYRO2) [MIM:276600]; also known as Richner-Hanhart syndrome. TYRO2 is an inborn error of metabolism characterized by elevations of tyrosine in the blood and urine, and oculocutaneous manifestations. Typical features include palmoplantar keratosis, painful corneal ulcers, and mental retardation.
    • Sequence similaritiesBelongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
    • Information by UniProt
    • Database links
    • Alternative names
      • ATTY_HUMAN antibody
      • L-tyrosine:2-oxoglutarate aminotransferase antibody
      • TAT antibody
      • Tyrosine aminotransferase antibody
      • Tyrosine aminotransferase, cytosolic antibody
      see all

    Anti-ATTY antibody [EPR6121] images

    • All lanes : Anti-ATTY antibody [EPR6121] (ab125000) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate, treated with insulin
      Lane 2 : HepG2 cell lysate, untreated
      Lane 3 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 50 kDa
    • ab125000 at 1/50 dilution staining ATTY in paraffin-embedded Human brain tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-ATTY antibody [EPR6121] (ab125000)

    This product has been referenced in:
    • Nandi SS  et al. Lack of miR-133a Decreases Contractility of Diabetic Hearts: A Role for Novel Cross Talk Between Tyrosine Aminotransferase and Tyrosine Hydroxylase. Diabetes 65:3075-90 (2016). Read more (PubMed: 27411382) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab125000.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"