Anti-AU5 tag antibody [AU5] (ab24576)

Overview

  • Product name
    Anti-AU5 tag antibody [AU5]
  • Description
    Mouse monoclonal [AU5] to AU5 tag
  • Tested applications
    Suitable for: WB, ICC/IF, IPmore details
  • Immunogen

    This antibody was raised against an SDS disrupted BPV1 preparation and mapped to the six amino acid epitope TDFYLK.

  • Epitope
    This antibody maps to the six amino acid epitope TDFYLK.
  • General notes


    This clone was specifically designed for use in epitope tagging. Because of its small size, clone AU5 is unlikely to alter the activity of the cloned sequence.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.03% Thimerosal (merthiolate)
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Primary antibody notes
    This clone was specifically designed for use in epitope tagging. Because of its small size, clone AU5 is unlikely to alter the activity of the cloned sequence.
  • Clonality
    Monoclonal
  • Clone number
    AU5
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab24576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 1 kDa.
ICC/IF 1/500.
IP 1/150.

Target

  • Relevance
    AU5 is a commonly used epitope tag engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized. Because of the small size of the epitope, it is unlikely to alter the activity of the cloned sequence.
  • Alternative names
    • AU5 epitope tag antibody
    • TDFYLK epitope tag antibody
    • TDFYLK tag antibody

References for Anti-AU5 tag antibody [AU5] (ab24576)

ab24576 has not yet been referenced specifically in any publications.

Product Wall

Application
Flow Cytometry
Sample
Mouse Cell (Yeast cell)
Permeabilization
No
Gating Strategy
Singlets of yeast
Specification
Yeast cell
Preparation
Cell harvesting/tissue preparation method: Activated in SG buffer
Sample buffer: PBSA
Fixation
None
Username

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Submitted Apr 25 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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