Recombinant Anti-AUH antibody [EPR11086(B)] (ab157453)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11086(B)] to AUH
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-AUH antibody [EPR11086(B)]
See all AUH primary antibodies -
Description
Rabbit monoclonal [EPR11086(B)] to AUH -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Mouse brain, Rat brain, Rat heart and Rat kidney cell lysates; SH-SY5Y cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11086(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab157453 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
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ICC/IF |
1/100 - 1/250.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 36 kDa. |
ICC/IF
1/100 - 1/250. |
Target
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Relevance
AUH (3-methylglutaconyl-CoA hydratase) catalyzes the conversion of 3-methylglutaconyl-CoA to 3-hydroxy-3-methylglutaryl-CoA and has very low enoyl-CoA hydratase activity. Deletion or mutation of the AUH gene causes the metabolic disease 3-methylglutaconic aciduria type I (MGA1). MGA type I is characterized by an abnormal organic acid profile in which there is excessive urinary excretion of 3-methylglutaconic acid, 3-methylglutaric acid and 3-hydroxyisovaleric acid. AUH is also an RNA-binding protein that binds in vitro to clustered 5'-AUUUA-3' motifs. -
Cellular localization
Mitochondrial -
Database links
- Entrez Gene: 549 Human
- Entrez Gene: 11992 Mouse
- Entrez Gene: 361215 Rat
- Omim: 600529 Human
- SwissProt: Q13825 Human
- SwissProt: Q9JLZ3 Mouse
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Alternative names
- 3 methylglutaconyl CoA hydratase antibody
- AU binding protein/enoyl CoA hydratase antibody
- AU RNA binding protein/enoyl CoA hydratase antibody
see all
Images
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All lanes : Anti-AUH antibody [EPR11086(B)] (ab157453) at 1/1000 dilution
Lane 1 : Mouse brain cell lysate
Lane 2 : Rat brain cell lysate
Lane 3 : Rat heart cell lysate
Lane 4 : Rat kidney cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 36 kDa -
Immunofluorescent analysis of SH-SY5Y cells labeling AUH with ab157453 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab157453 has been referenced in 3 publications.
- Son SM et al. Leucine regulates autophagy via acetylation of the mTORC1 component raptor. Nat Commun 11:3148 (2020). PubMed: 32561715
- Son SM et al. Leucine Signals to mTORC1 via Its Metabolite Acetyl-Coenzyme A. Cell Metab 29:192-201.e7 (2019). PubMed: 30197302
- Zhang Y et al. PINK1 Inhibits Local Protein Synthesis to Limit Transmission of Deleterious Mitochondrial DNA Mutations. Mol Cell 73:1127-1137.e5 (2019). PubMed: 30772175