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Immunogen was a synthetic peptide, which represented a C-terminal domain of mouse Aurora Kinase A (LocusLink ID 20878).
Our Abpromise guarantee covers the use of ab12324 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent dilution.|
|WB||1/2000 - 1/15000. Predicted molecular weight: 48 kDa.|
Detection of Rat Aurora A by Western Blot. Samples: Whole tissue lysate (60 mcg/lane) from rat mammary glands. Antibody: ab12324 used at 0.2 mcg/ml. Detection: Chemiluminescence with an exposure time of 6 minutes. *Note: The upper band is believed to be phosphorylated Aurora A.
ab12324 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"