• Product nameAnti-Aurora A antibody
    See all Aurora A primary antibodies
  • Description
    Rabbit polyclonal to Aurora A
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide (Human) conjugated to KLH - which represented a portion of human Serine/Threonine Kinase 15 encoded within exon 5 (LocusLink ID 8465)



Our Abpromise guarantee covers the use of ab1287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. Used at a dilution of 1/1000 for 1 hr on HeLa cells (see Abreview).
IHC-P 1/250 - 1/1000.
WB 1/4000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast, pancreas and cervical cancer cell lines. Expression is cell-cycle regulated, low in G1/S, accumulates during G2/M, and decreases rapidly after.
IP Use a concentration of 2 - 10 µg/ml.


  • FunctionContributes to the regulation of cell cycle progression. Required for normal mitosis. Associates with the centrosome and the spindle microtubules during mitosis and functions in centrosome maturation, spindle assembly, maintenance of spindle bipolarity, centrosome separation and mitotic checkpoint control. Phosphorylates numerous target proteins, including ARHGEF2, BRCA1, KIF2A, NDEL1, PARD3, PLK1 and BORA. Regulates KIF2A tubulin depolymerase activity (By similarity). Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization.
  • Tissue specificityHighly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    Activated by phosphorylation at Thr-288; this brings about a change in the conformation of the activation segment. Phosphorylation at Thr-288 varies during the cell cycle and is highest during M phase. Autophosphorylated at Thr-288 upon TPX2 binding. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated by CHFR, leading to its degradation by the proteasome (By similarity). Ubiquitinated by the anaphase-promoting complex (APC), leading to its degradation by the proteasome.
  • Cellular localizationCytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Detected at the neurite hillock in developing neurons (By similarity). Localizes on centrosomes in interphase cells and at each spindle pole in mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • AIK antibody
    • ARK-1 antibody
    • ARK1 antibody
    • AURA antibody
    • Aurka antibody
    • Aurora 2 antibody
    • Aurora A antibody
    • Aurora kinase A antibody
    • Aurora-related kinase 1 antibody
    • Aurora/IPL1 like kinase antibody
    • Aurora/IPL1-related kinase 1 antibody
    • AURORA2 antibody
    • Breast tumor-amplified kinase antibody
    • BTAK antibody
    • hARK1 antibody
    • IAK antibody
    • IPL1 related kinase antibody
    • MGC34538 antibody
    • OTTHUMP00000031340 antibody
    • OTTHUMP00000031341 antibody
    • OTTHUMP00000031342 antibody
    • OTTHUMP00000031343 antibody
    • OTTHUMP00000031344 antibody
    • OTTHUMP00000031345 antibody
    • OTTHUMP00000166071 antibody
    • OTTHUMP00000166072 antibody
    • PPP1R47 antibody
    • Protein phosphatase 1, regulatory subunit 47 antibody
    • Serine/threonine kinase 15 antibody
    • Serine/threonine kinase 6 antibody
    • Serine/threonine-protein kinase 15 antibody
    • Serine/threonine-protein kinase 6 antibody
    • Serine/threonine-protein kinase aurora-A antibody
    • STK15 antibody
    • STK6 antibody
    • STK6_HUMAN antibody
    • STK7 antibody
    see all

Anti-Aurora A antibody images

  • Immunoprecipitation of Human Aurora A.
    35S-Met labeled whole cell lysate from cells transfected with a human Aurora A expression construct. ab1287 was used at 4 µg/ml for IP. Detection: Autoradiography.
  • ab1287 (1/250) detecting Aurora A in paraffin embedded Human pancreatic tumour tissue sections. Detection: DAB staining;
  • ab1287 at a 1/1000 dilution staining synchronised HeLa cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with 1% serum prior to incubation with the primary antibody for 1 hour. A Cy5 conjugated donkey anti-rabbit antibody was used as the secondary. The image shows a DAPI panel, Aurora-A panel, and merge panel of DAPI (blue), microtubules (red) and Aurora-A (green).

    See Abreview

  • Anti-Aurora A antibody (ab1287) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 48 kDa
    Observed band size : 48 kDa
    Additional bands at : 20 kDa,25 kDa,28 kDa,37 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Aurora A antibody (ab1287)

This product has been referenced in:
  • Diaz A  et al. TERT and AURKA gene copy number gains enhance the detection of acral lentiginous melanomas by fluorescence in situ hybridization. J Mol Diagn 16:198-206 (2014). IHC . Read more (PubMed: 24374110) »
  • Mbom BC  et al. Nek2 phosphorylates and stabilizes ß-catenin at mitotic centrosomes downstream of Plk1. Mol Biol Cell 25:977-91 (2014). WB ; Human . Read more (PubMed: 24501426) »

See all 15 Publications for this product

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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In general, we recommend the tissue you see on the datasheet, in this case a pancreatic tumor. We offer sections of pancreatic adenocarcinoma, not of the exact same tumor, but we assume the staining will be similar. Here is a link to the datasheet for ...

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Thank you for your enquiry and your interest in our products.

I can confirm that we have 9 vials of batch: GR4766-7 in our UK stock.

I hope this helps and if I can assist further, please do not hesitate to contact me.

Thank you for your enquiry. I am afraid your image did not come through. All of our antibodies are stable at room temperature for at least 2 weeks. From the information you have given, I cannot tell whether there may be something in your protoco...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%

Mr. Michael Porter

Verified customer

Submitted Aug 14 2006

Thank you for your enquiry. For ab1287, the originator has recommended using 0.1 M citrate buffer, pH 6.6 and 1mM EDTA. For ab2254, we would suggest microwave pre-treatment in citrate buffer (pH 6.0) for 5 mins high then 5 mins simmer (800W conventio...

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I'm afraid the precise immunogen sequence used is proprietary. The binding site is between residues 50 and 100 of human Aurora A using the numbering given in entry NP_003591.1. I hope this information helps,

Thank you for your email. The immunogen used was a synthetic peptide (Human) conjugated to KLH - which represented a portion of human Serine/Threonine Kinase 15 encoded within exon 5 (LocusLink ID 8465). If you have any more questions, please contact u...

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Thank you for your enquiry. The buffer is 50 mM Tris, 5 mM Citrate and 40 mM Phosphate. If you need any further assistance please do not hesitate to get back in touch.

Thank you for your enquiry and for sending us your detailed protocol. We would suggest blocking the non-specific binding sites with 1%BSA for 1 hr and incubating the samples with the primary antibody for 2 hrs (40 min is too short). This antibo...

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