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Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Aurora A.
(Peptide available as ab13008.)
Our Abpromise guarantee covers the use of ab12875 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Metaphase HeLa cells stained with ab12875 and counterstained with DAPI. The antibody shows the localisation of Aurora A to the centrosome during metaphase. 100x magnification.
Panel 1: DNA stained with DAPI.
Panel 2: Cells stained with ab12875 (1/500 dilution).
Panel 3: DAPI staining is shown in red and ab12875 in green.
The image shows staining of formalin fixed paraffin embedded human tonsil sections with ab12875 (1/20 working dilution). Clear nuclear staining can be seen.