Anti-Aurora A antibody - Centrosome Marker (ab12875)

Overview

  • Product nameAnti-Aurora A antibody - Centrosome Marker
    See all Aurora A primary antibodies
  • Description
    Rabbit polyclonal to Aurora A - Centrosome Marker
  • Specificityab12875 detects a single clean band of just below 50kD in Jurkat and Hela cell lysates. This band corresponds to the size of Aurora A seen using other antibodies.
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IF, ICCmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Aurora A.

    (Peptide available as ab13008.)

  • Positive control
    • Jurkat cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab12875 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
WB
ICC/IF
ICC
  • Application notesICC: 1/500.
    IF: 1/500.
    IHC-P: 1/15 - 1/20. Antigen retrieval was performed using either Sodium Citrate or Tris/EDTA before commencing with the IHC staining protocol. Customer feedback suggests that a 1/15 dilution of ab12875 is recommended when using the sodium citrate antigen retreival method and a 1/20 dilution is recommended when using the Tris/EDTA antigen retrieval method.

    WB: 1/500. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionContributes to the regulation of cell cycle progression. Required for normal mitosis. Associates with the centrosome and the spindle microtubules during mitosis and functions in centrosome maturation, spindle assembly, maintenance of spindle bipolarity, centrosome separation and mitotic checkpoint control. Phosphorylates numerous target proteins, including ARHGEF2, BRCA1, KIF2A, NDEL1, PARD3, PLK1 and BORA. Regulates KIF2A tubulin depolymerase activity (By similarity). Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization.
    • Tissue specificityHighly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.
    • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Activated by phosphorylation at Thr-288; this brings about a change in the conformation of the activation segment. Phosphorylation at Thr-288 varies during the cell cycle and is highest during M phase. Autophosphorylated at Thr-288 upon TPX2 binding. Phosphorylated upon DNA damage, probably by ATM or ATR.
      Ubiquitinated by CHFR, leading to its degradation by the proteasome (By similarity). Ubiquitinated by the anaphase-promoting complex (APC), leading to its degradation by the proteasome.
    • Cellular localizationCytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Detected at the neurite hillock in developing neurons (By similarity). Localizes on centrosomes in interphase cells and at each spindle pole in mitosis.
    • Information by UniProt
    • Database links
    • Alternative names
      • AIK antibody
      • ARK-1 antibody
      • ARK1 antibody
      • AURA antibody
      • Aurka antibody
      • Aurora 2 antibody
      • Aurora A antibody
      • Aurora kinase A antibody
      • Aurora-related kinase 1 antibody
      • Aurora/IPL1 like kinase antibody
      • Aurora/IPL1-related kinase 1 antibody
      • AURORA2 antibody
      • Breast tumor-amplified kinase antibody
      • BTAK antibody
      • hARK1 antibody
      • IAK antibody
      • IPL1 related kinase antibody
      • MGC34538 antibody
      • OTTHUMP00000031340 antibody
      • OTTHUMP00000031341 antibody
      • OTTHUMP00000031342 antibody
      • OTTHUMP00000031343 antibody
      • OTTHUMP00000031344 antibody
      • OTTHUMP00000031345 antibody
      • OTTHUMP00000166071 antibody
      • OTTHUMP00000166072 antibody
      • PPP1R47 antibody
      • Protein phosphatase 1, regulatory subunit 47 antibody
      • Serine/threonine kinase 15 antibody
      • Serine/threonine kinase 6 antibody
      • Serine/threonine-protein kinase 15 antibody
      • Serine/threonine-protein kinase 6 antibody
      • Serine/threonine-protein kinase aurora-A antibody
      • STK15 antibody
      • STK6 antibody
      • STK6_HUMAN antibody
      • STK7 antibody
      see all

    Anti-Aurora A antibody - Centrosome Marker images

    • All lanes : Anti-Aurora A antibody - Centrosome Marker (ab12875) at 1/500 dilution

      Lane 1 : Jurkat whole cell lysate
      Lane 2 : HeLa whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Alexa Fluor 680 Goat anti Rabbit IgG at 1/5000 dilution

      Performed under reducing conditions.

      Predicted band size : 46 kDa
      Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    • Metaphase HeLa cells stained with ab12875 and counterstained with DAPI. The antibody shows the localisation of Aurora A to the centrosome during metaphase. 100x magnification.

      Panel 1: DNA stained with DAPI.
      Panel 2: Cells stained with ab12875 (1/500 dilution).
      Panel 3: DAPI staining is shown in red and ab12875 in green.

    • The image shows staining of formalin fixed paraffin embedded human tonsil sections with ab12875 (1/20 working dilution). Clear nuclear staining can be seen.

    References for Anti-Aurora A antibody - Centrosome Marker (ab12875)

    This product has been referenced in:
    • Hammond D  et al. Melanoma-associated mutations in protein phosphatase 6 cause chromosome instability and DNA damage owing to dysregulated Aurora-A. J Cell Sci 126:3429-40 (2013). Read more (PubMed: 23729733) »
    • Morita E  et al. Human ESCRT-III and VPS4 proteins are required for centrosome and spindle maintenance. Proc Natl Acad Sci U S A 107:12889-94 (2010). ICC/IF ; Human . Read more (PubMed: 20616062) »

    See all 4 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris gel)
    Sample Human Cell lysate - whole cell (U2OS-osteosarcoma)
    Specification U2OS-osteosarcoma
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Dr. Carlos le Sage

    Verified customer

    Submitted Jan 21 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa)
    Loading amount 20 µg
    Specification HeLa
    Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 15°C
    Username

    Ms. AeLee Jeong

    Verified customer

    Submitted Apr 04 2013

    Thank you for your reply.

    I apologize for the confusion, there was a difference in the numerical order of amino acids listed in the alignment I was performing. You are correct, ab115883 should not be chosen as the immunogen sequence does not o...

    Read More

    Thank you for contacting Abcam.

    We discussed potential anti-Aurora A antibodies to detect Chlamydomonas reinhardtii Aurora A. Since C. reinhardtii Aurora A has 67.2% homology with human Aurora A, this does not qualify for our AbTrial program, ...

    Read More

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

    Read More

    Thank you for your reply.

    I would be happy to offer you a replacement antibody or a credit. Please let me know which you would prefer. If you would like an antibody, please include the catalogue number.

    I look forward to your reply.

    Thank you for taking time to contact us. I am sorry to hear that this antibody is not providing satisfactory results.

    The details provided will enable us to investigate this case and will provide us with vital information for monitoring prod...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cultured Cells (Head and neck cancer cell)
    Specification Head and neck cancer cell
    Fixative Acetone
    Permeabilization No
    Blocking step FBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 27°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 04 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Bladder)
    Specification Bladder
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
    Username

    Abcam user community

    Verified customer

    Submitted Jan 23 2007

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry
    Sample Human Cell (HeLa and SK-N-SH)
    Specification HeLa and SK-N-SH
    Fixative Paraformaldehyde
    Username

    Dr. Kirk McManus

    Verified customer

    Submitted Oct 12 2005

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"