Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)


  • Product nameAnti-Aurora A antibody [EPR5026] - Centrosome Marker
    See all Aurora A primary antibodies
  • Description
    Rabbit monoclonal [EPR5026] to Aurora A - Centrosome Marker
  • Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human Aurora A

  • Positive control
    • BXPC-3, LnCaP, SKBR-3 and HepG2 cell lysates. HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab108353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 46 kDa.
Flow Cyt 1/1000 - 1/10000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for IHC-P or IP.
  • Target

    • FunctionContributes to the regulation of cell cycle progression. Required for normal mitosis. Associates with the centrosome and the spindle microtubules during mitosis and functions in centrosome maturation, spindle assembly, maintenance of spindle bipolarity, centrosome separation and mitotic checkpoint control. Phosphorylates numerous target proteins, including ARHGEF2, BRCA1, KIF2A, NDEL1, PARD3, PLK1 and BORA. Regulates KIF2A tubulin depolymerase activity (By similarity). Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization.
    • Tissue specificityHighly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.
    • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      Activated by phosphorylation at Thr-288; this brings about a change in the conformation of the activation segment. Phosphorylation at Thr-288 varies during the cell cycle and is highest during M phase. Autophosphorylated at Thr-288 upon TPX2 binding. Phosphorylated upon DNA damage, probably by ATM or ATR.
      Ubiquitinated by CHFR, leading to its degradation by the proteasome (By similarity). Ubiquitinated by the anaphase-promoting complex (APC), leading to its degradation by the proteasome.
    • Cellular localizationCytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Detected at the neurite hillock in developing neurons (By similarity). Localizes on centrosomes in interphase cells and at each spindle pole in mitosis.
    • Information by UniProt
    • Database links
    • Alternative names
      • AIK antibody
      • ARK-1 antibody
      • ARK1 antibody
      • AURA antibody
      • Aurka antibody
      • Aurora 2 antibody
      • Aurora A antibody
      • Aurora kinase A antibody
      • Aurora-related kinase 1 antibody
      • Aurora/IPL1 like kinase antibody
      • Aurora/IPL1-related kinase 1 antibody
      • AURORA2 antibody
      • Breast tumor-amplified kinase antibody
      • BTAK antibody
      • hARK1 antibody
      • IAK antibody
      • IPL1 related kinase antibody
      • MGC34538 antibody
      • OTTHUMP00000031340 antibody
      • OTTHUMP00000031341 antibody
      • OTTHUMP00000031342 antibody
      • OTTHUMP00000031343 antibody
      • OTTHUMP00000031344 antibody
      • OTTHUMP00000031345 antibody
      • OTTHUMP00000166071 antibody
      • OTTHUMP00000166072 antibody
      • PPP1R47 antibody
      • Protein phosphatase 1, regulatory subunit 47 antibody
      • Serine/threonine kinase 15 antibody
      • Serine/threonine kinase 6 antibody
      • Serine/threonine-protein kinase 15 antibody
      • Serine/threonine-protein kinase 6 antibody
      • Serine/threonine-protein kinase aurora-A antibody
      • STK15 antibody
      • STK6 antibody
      • STK6_HUMAN antibody
      • STK7 antibody
      see all

    Anti-Aurora A antibody [EPR5026] - Centrosome Marker images

    • Overlay histogram showing HeLa cells stained with ab108353 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108353, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • All lanes : Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353) at 1/1000 dilution

      Lane 1 : BXPC-3 cell lysate
      Lane 2 : LnCaP cell lysate
      Lane 3 : SKBR-3 cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 46 kDa
    • Immunofluorescent staining of HeLa cells using ab108353 at 1/100
    • Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353) at 1/1000 dilution + Neuro-2a cell lysate at 10 µg

      Predicted band size : 46 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-Aurora A antibody [EPR5026] - Centrosome Marker (ab108353)

    ab108353 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris gel)
    Sample Human Cell lysate - whole cell (U2OS-osteosarcoma)
    Specification U2OS-osteosarcoma
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

    Dr. Carlos le Sage

    Verified customer

    Submitted Jan 21 2014

    Thank you for your reply.

    I apologize for the confusion, there was a difference in the numerical order of amino acids listed in the alignment I was performing. You are correct, ab115883 should not be chosen as the immunogen sequence does not o...

    Read More