Anti-Aurora B antibody [mAbcam 3609] (ab3609)
Key features and details
- Mouse monoclonal [mAbcam 3609] to Aurora B
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Aurora B antibody [mAbcam 3609]
See all Aurora B primary antibodies -
Description
Mouse monoclonal [mAbcam 3609] to Aurora B -
Host species
Mouse -
Specificity
Based on the immunogen sequence, we do not expect the antibody to cross-react with AURKA or AURKC. No cross-reactivity testing has been performed.
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Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Hela whole cell lysate
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
mAbcam 3609 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3609 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). Block with 1% BSA for 1 hour.
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IHC-P |
Use at an assay dependent concentration.
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). Block with 1% BSA for 1 hour. |
IHC-P
Use at an assay dependent concentration. |
Target
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Function
May be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis. Required for kinetochore localization of BUB1 and SGOL1. Interacts with INCENP. -
Tissue specificity
High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase. -
Involvement in disease
Note=Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsUbiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome. -
Cellular localization
Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body. - Information by UniProt
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Database links
- Entrez Gene: 9212 Human
- Entrez Gene: 20877 Mouse
- Entrez Gene: 114592 Rat
- Omim: 604970 Human
- SwissProt: Q96GD4 Human
- SwissProt: O70126 Mouse
- SwissProt: O55099 Rat
- Unigene: 442658 Human
see all -
Alternative names
- AIK2 antibody
- AIM-1 antibody
- AIM1 antibody
see all
Images
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All lanes : Anti-Aurora B antibody [mAbcam 3609] (ab3609) at 1 µg/ml
Lane 1 : Hek293 Whole Cell Lysate
Lane 2 : Jurkat Whole Cell Lysate
Lane 3 : HeLa Whole Cell Lysate
Lane 4 : HeLa Whole Cell Lysate- Nocodozole Stimulated
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Absorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes -
Image courtesy of Human Protein Atlas
Paraffin embedded human liver tumor tissue was incubated with ab3609 (1/35 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab3609 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further images can be found at www.proteinatlas.org
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (27)
ab3609 has been referenced in 27 publications.
- Joshi J et al. Optogenetic Control of Engrafted Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes in Live Mice: A Proof-of-Concept Study. Cells 11:N/A (2022). PubMed: 35326403
- Khatib JB et al. Complementary CRISPR genome-wide genetic screens in PARP10-knockout and overexpressing cells identify synthetic interactions for PARP10-mediated cellular survival. Oncotarget 13:1078-1091 (2022). PubMed: 36187556
- Wu Y et al. LRP6 downregulation promotes cardiomyocyte proliferation and heart regeneration. Cell Res 31:450-462 (2021). PubMed: 32973339
- Fan C et al. Myocardial protection by nanomaterials formulated with CHIR99021 and FGF1. JCI Insight 5:N/A (2020). PubMed: 32453715
- Sharp JA et al. Cell division requires RNA eviction from condensing chromosomes. J Cell Biol 219:N/A (2020). PubMed: 33053167