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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Aurora B.
(Peptide available as ab13569.)
Our Abpromise guarantee covers the use of ab3609 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). Block with 1% BSA for 1 hour.|
|IHC-P||Use at an assay dependent concentration.|
SKN-SH cells were fixed in 4% paraformaldehyde for 10 mins, permeabilized in PBS-0.5% Triton X-100 for 5 mins and incubated for 30 minutes with ab3609 (1/50 dilution). The slides were blocked with 4mg/ml BSA. The slides were rinsed once in PBS-Triton (0.1%), twice in PBS then incubated with the secondary antibody for 30 mins. The DNA is stained with DAPI (blue). Staining with ab3609 can be seen in green.
Paraffin embedded human liver tumor tissue was incubated with ab3609 (1/35 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab3609 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further images can be found at www.proteinatlas.org