Overview

  • Product nameAnti-AUTS2 antibody
  • Description
    Rabbit polyclonal to AUTS2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant protein fragment contain a sequence corresponding to a region within amino acids 524 and 770 of AUTS2

  • Positive control
    • 293T and NIH-3T3 cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 139 kDa.

Target

Anti-AUTS2 antibody images

  • Anti-AUTS2 antibody (ab96326) at 1/1000 dilution + 293T cell lysate at 30 µg

    Predicted band size : 139 kDa
  • Anti-AUTS2 antibody (ab96326) at 1/1000 dilution + NIH3T3 cell lysate at 30 µg

    Predicted band size : 139 kDa

References for Anti-AUTS2 antibody (ab96326)

This product has been referenced in:
  • Oksenberg N  et al. Genome-wide distribution of Auts2 binding localizes with active neurodevelopmental genes. Transl Psychiatry 4:e431 (2014). ChIP ; Mouse . Read more (PubMed: 25180570) »

See 1 Publication for this product

Product Wall

Unfortunately this is the only buffer formulation available for this product at present. You may remove the glycerol by use of an Antibody Concentration Kit (ab102776). Dilute the antibody to reduce the glycerol’s concentration, which in turn will mak...

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Thank you for your reply and for providing the protocol information.

Your protocol looks ok to me and so should be fine.

I just had a few other questions, have you done a no primary control? Also now that I look at your blot again, ...

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Thank you for contacting Abcam.

The antibody, ab25788, is covered under our Abpromise for six months and is guaranteed to work in western blot on mouse and human samples. We have not yet tested the antibody in IHC and therefore it may not be ...

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Thank you for that information.


Can I just clarify something. You mentioned that the gel was probed twice, onceusing 1% milk in 1x PBS 0.3%Tween.This was then restained using5% milk in 1xTBS 0.05%Tween. For the first condition did you s...

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Thank you for calling us and for alerting us to the problem you are experiencing with Anti-AUTS2 antibodyab96326. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

As we di...

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Thank you for contacting us. The immunogen that was used to create ab96326 shows 100% identity with human long isoform of AUTS2 and 89% identity with short isoform of AUTS2. So this antibody will be detecting both the isoforms. I hope this info...

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Thank you for contacting us. I have read the whole protocol you have kindly submitted. In order to address the problem quickly I have further questions to ask. - According to the attached image you have used non transfected N2a mouse cells, Lym...

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Thank you for your email. I can confirm that I added the discount code to our internal documents of the Abreview 26773. Your customer can now redeem this discount code and order a free antibody. Please thank your customer for he...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - nuclear (N2a mouse neuroblastoma cells)
Loading amount 150000 cells
Specification N2a mouse neuroblastoma cells
Treatment lysed in radioimmunoprecipitation (RIPA) buffer (150 mM NaCl, 1% Triton X-100, 1% sodium deoxycholate, 50 mM Tris-HCl, pH 7.5, 2 mM EDTA) supplemented with protease inhibitor cocktail for 20 min. on ice
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Dr. Laura Malki Feldman

Verified customer

Submitted Nov 03 2011

Thank you for your recent enquiry which has been forwarded to me as my colleague is currently away from the office. I can confirm that ab96326 Anti-AUTS2 antibody was used to detect endogenous AUTS2 in 293T and NIH3T3 cells in the images on the da...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"