Anti-Avian Influenza A Hemagglutinin 3 antibody (ab62485)

Overview

  • Product nameAnti-Avian Influenza A Hemagglutinin 3 antibody
  • Description
    Goat polyclonal to Avian Influenza A Hemagglutinin 3
  • SpecificityThis antibody reacts with the Hemagglutinin 3 protein from the H5N1 strain of Avian Influenza A
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with avian influenza A
  • Immunogen

    Synthetic peptide corresponding to 14 amino acids near the center of the Hemagglutinin 3 protein from the H5N1 strain of Avian Influenza A

  • Positive control
    • Recombinant Hemagglutinin 3

Properties

Applications

Our Abpromise guarantee covers the use of ab62485 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: Use at a concentration of 1 µg/ml. This antibody will detect 10 ng of free peptide at 1 µg/ml.
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 47 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceInfluenza A Hemagglutinin 3 binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induce an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore. The extent of infection into host organism is determined by HA. Influenza viruses bud from the apical surface of polarized epithelial cells (e.g. bronchial epithelial cells) into lumen of lungs and are therefore usually pneumotropic. The reason is that HA is cleaved by tryptase clara which is restricted to lungs. However, HAs of H5 and H7 pantropic avian viruses subtypes can be cleaved by furin and subtilisin-type enzymes, allowing the virus to grow in other organs than lungs.
    • Alternative names
      • H3 antibody
      • Hemagglutinin 3 antibody
      • Hemagglutinin antibody

    Anti-Avian Influenza A Hemagglutinin 3 antibody images

    • All lanes : Anti-Avian Influenza A Hemagglutinin 3 antibody (ab62485) at 1 µg/ml

      Lane 1 : 1ng of Recombinant Hemagglutinin
      Lane 2 : 5ng of Recombinant Hemagglutinin

      Secondary
      Anti goat IgG secondary antibody

      Observed band size : 47 kDa (why is the actual band size different from the predicted?)

    References for Anti-Avian Influenza A Hemagglutinin 3 antibody (ab62485)

    ab62485 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your enquiry. I have enquired at the originator of the product. Unfortunately, the immunogen sequence is proprietary, but after running a BLAST search, they have confirmed that it shows 100% homology with the China sequence. I hope...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"