Anti-Avian Influenza A Hemagglutinin antibody (ab62587)
Key features and details
- Goat polyclonal to Avian Influenza A Hemagglutinin
- Suitable for: ELISA, WB
- Isotype: IgG
Overview
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Product name
Anti-Avian Influenza A Hemagglutinin antibody
See all Avian Influenza A Hemagglutinin primary antibodies -
Description
Goat polyclonal to Avian Influenza A Hemagglutinin -
Host species
Goat -
Specificity
ab62587 can be used for the detection of the Hemagglutinin protein from the H5N1 strain of avian influenza A in ELISA and WB. It will detect 10 ng of free peptide at 1 μg/mL.
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Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: H5N1 subtype -
Immunogen
Synthetic peptide corresponding to 12 amino acids near the amino terminus of the Avian influenza A Hemagglutinin 1 protein. Efforts were made to use relatively conserved regions of the viral sequence as the antigen.
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Positive control
- WB: H5N1 Hemagglutinin recombinant protein. ELISA: H5N1 Hemagglutinin recombinant protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab62587 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA | ||
WB |
Notes |
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WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 64 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Relevance
Influenza A virus is a major public health threat, killing more than 30,000 people per year in the USA. Novel influenza virus strains caused by genetic drift and viral recombination emerge periodically to which humans have little or no immunity, resulting in devastating pandemics. Influenza A can exist in a variety of animals; however it is in birds that all subtypes can be found. These subtypes are classified based on the combination of the virus coat glycoproteins hemagglutinin (HA) and neuraminidase (NA) subtypes. HA interacts with cell surface proteins containing oligosaccharides with terminal sialyl residues. Virus isolated from a human infected with the H5N1 strain in 1997 could bind to oligosaccharides from human as well as avian sources, indicating its species-jumping ability. -
Cellular localization
Cell Membrane -
Alternative names
- HA1 antibody
- HA2 antibody
- Hemagglutinin antibody
see all
Images
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Lane 1 : Anti-Avian Influenza A Hemagglutinin antibody (ab62587) at 0.5 µg/ml
Lane 2 : Anti-Avian Influenza A Hemagglutinin antibody (ab62587) at 1 µg/ml
All lanes : 30 ng of H5N1 Hemagglutinin recombinant protein
Secondary
All lanes : Rabbit anti-goat IgG HRP conjugate at 1/10000 dilution
Predicted band size: 64 kDa1h incubation at RT in 5% NFDM/TBST.
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Validation with H5N1 HA Protein Coating Antigen: H5N1 Hemagglutinin recombinant protein, 2 μg/mL, incubated at 4 ˚C overnight. Detection Antibodies: ab62587, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Rabbit anti-goat HRP at 1/10000, incubated at RT for 1 hr.
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All lanes : Anti-Avian Influenza A Hemagglutinin antibody (ab62587) at 1 µg/ml
Lane 1 : 5 ng of Recombinant HA1
Lane 2 : 25 ng of Recombinant HA1
Predicted band size: 64 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Additional bands at: 33 kDa, 35 kDa, 40 kDa. We are unsure as to the identity of these extra bands.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab62587 has not yet been referenced specifically in any publications.