Anti-Avian Influenza Nucleoprotein antibody (ab22285)

Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 0.05% BSA, PBS
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab22285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.1 µg/ml.
ICC/IF 1/10.
WB Use at an assay dependent concentration. PubMed: 23538443

Target

  • RelevanceEncapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus
  • Cellular localizationNuclear
  • Alternative names
    • NP antibody
    • Nucleocapsid protein antibody
    • Nucleoprotein antibody
    • Protein N antibody

Anti-Avian Influenza Nucleoprotein antibody images

  • Immunofluoroscence staining of influenza−infected MDCK cells using ab22285 at 1:10 dilution.

    Image Courtesy of Catherine Thompson, The University of Reading

References for Anti-Avian Influenza Nucleoprotein antibody (ab22285)

This product has been referenced in:
  • Tripathi S  et al. Influenza A virus nucleoprotein induces apoptosis in human airway epithelial cells: implications of a novel interaction between nucleoprotein and host protein Clusterin. Cell Death Dis 4:e562 (2013). WB . Read more (PubMed: 23538443) »
  • Yamamoto Y  et al. Detecting avian influenza virus (H5N1) in domestic duck feathers. Emerg Infect Dis 14:1671-2 (2008). Read more (PubMed: 18826847) »

See all 2 Publications for this product

Product Wall

Thank you for your inquiry. The immunogen was a synthetic peptide corresponding to amino acids 428-441 (AFTGNTEGRTSDMR) of avian flu nucleoprotein (GenBank ADC34563.1). I hope this information is helpful to you. Please do not hesitate to contact...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"