Anti-Avian Influenza Nucleoprotein antibody (ab25921)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab25921 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 0.5-2.0 µg/ml.

    Please note from the image legend that a recombinant fusion protein was used to generate the western blot image.

    The molecular weight shown is the molecular weight of the fusion protein (e.g nucleoprotein amino acids 58-77 + fusion protein) not the Avian Influenza Nucleoprotein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceEncapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus
    • Cellular localizationNuclear
    • Alternative names
      • NP antibody
      • Nucleocapsid protein antibody
      • Nucleoprotein antibody
      • Protein N antibody

    Anti-Avian Influenza Nucleoprotein antibody images

    • All lanes : Anti-Avian Influenza Nucleoprotein antibody (ab25921) at 0.5 µg/ml

      Lane 1 : Recombinant fusion protein containing amino acids 58-77 of Avian flu nucleoprotein at 0.1 µg
      Lane 2 : Fusion partner without these amino acids


      Observed band size : 31 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 56 kDa,59 kDa,66 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-Avian Influenza Nucleoprotein antibody (ab25921)

    ab25921 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for getting back to me. Please can you tell me what you mean by a protective antibody? Do you mean does it counteract an avian flu infection? I appreciate any clarity that you can add to this enquiry. I look forward to hearing from you.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"