Anti-Avidin antibody - Azide free (ab34824)

Overview

  • Product nameAnti-Avidin antibody - Azide free
    See all Avidin primary antibodies
  • Description
    Rabbit polyclonal to Avidin - Azide free
  • SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Avidin [Hen Egg White]. Cross reactivity against Avidin from other tissues may occur but have not been specifically determined.
  • Tested applicationsSuitable for: ELISA, WB, Dot blot, IPmore details
  • Species reactivity
    Reacts with: Chicken
  • Immunogen

    Full length protein (Chicken) - from Hen egg white.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by a delipidation and defibrination.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
Dot blot
IP
  • Application notesDot: Use at an assay dependent dilution.
    ELISA: 1/20,000 - 1/100,000.
    IP: Use at an assay dependent dilution.
    WB: 1/2000 - 1/10,000. Predicted molecular weight: 17 kDa.
    Suitable for most immunological methods requiring high titer and specificity.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionThe biological function of avidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of avidin).
    • Tissue specificitySynthesized in hen oviduct and concentrated in egg white (where it represent 0.05% of the total protein).
    • Sequence similaritiesBelongs to the avidin/streptavidin family.
      Contains 1 avidin-like domain.
    • Post-translational
      modifications
      N-linked glycan at Asn-41 consists of GlcNAc(beta1-2)Man(alpha1-3)[GlcNAc(beta1-4)][Man(alpha1-?)Man(alpha1-6)] Man(beta1-4)GlcNAc(beta1-4)GlcNAc.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • AVD antibody
      • AVID_CHICK antibody
      • Avidin antibody
      • LOC396260 antibody

    References for Anti-Avidin antibody - Azide free (ab34824)

    ab34824 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"