Overview

  • Product nameAnti-Avidin antibody (Biotin)
    See all Avidin primary antibodies
  • Description
    Rabbit polyclonal to Avidin (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: WB, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopymore details
  • Immunogen

    Avidin [Hen Egg White]

  • General notes

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per rabbit IgG molecule.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.60
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7235 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Dot blot
ELISA
IHC-P
IHC-Fr
Immunomicroscopy
  • Application notesImmunoblotting, ELISA (1:8,000 to 1:32,000), immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates.
  • Target

    • FunctionThe biological function of avidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of avidin).
    • Tissue specificitySynthesized in hen oviduct and concentrated in egg white (where it represent 0.05% of the total protein).
    • Sequence similaritiesBelongs to the avidin/streptavidin family.
      Contains 1 avidin-like domain.
    • Post-translational
      modifications
      N-linked glycan at Asn-41 consists of GlcNAc(beta1-2)Man(alpha1-3)[GlcNAc(beta1-4)][Man(alpha1-?)Man(alpha1-6)] Man(beta1-4)GlcNAc(beta1-4)GlcNAc.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Alternative names
      • AVD antibody
      • AVID_CHICK antibody
      • Avidin antibody
      • LOC396260 antibody

    References for Anti-Avidin antibody (Biotin) (ab7235)

    ab7235 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab7235.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"