Avidin Peroxidase Conjugate (ab59653)

Overview

Description

  • NatureRecombinant
  • SourceNative
  • Amino Acid Sequence
    • SpeciesChicken
  • ConjugationHRP

Specifications

Our Abpromise guarantee covers the use of ab59653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Western blot

    ELISA

  • FormLiquid
  • Additional notes


    Avidin-HRP conjugates are useful for detection, identification, quantitation, and localization of biotinylated substances.

    Reacts with biotinylated substances.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 0.1% BSA, PBS

General Info

  • Alternative names
    • AVD
    • AVID_CHICK
    • Avidin
    • LOC396260
  • FunctionThe biological function of avidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of avidin).
  • Tissue specificitySynthesized in hen oviduct and concentrated in egg white (where it represent 0.05% of the total protein).
  • Sequence similaritiesBelongs to the avidin/streptavidin family.
    Contains 1 avidin-like domain.
  • Post-translational
    modifications
    N-linked glycan at Asn-41 consists of GlcNAc(beta1-2)Man(alpha1-3)[GlcNAc(beta1-4)][Man(alpha1-?)Man(alpha1-6)] Man(beta1-4)GlcNAc(beta1-4)GlcNAc.
  • Cellular localizationSecreted.
  • Information by UniProt

References for Avidin Peroxidase Conjugate (ab59653)

ab59653 has not yet been referenced specifically in any publications.

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I can confirm that ab6675 has been tested in indirect ELISA; we do not have any data if it works together with ab59653 in Sandwich ELISA (sELISA) system. Unfortunately, we do not h...

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