Anti-AVL9 antibody - C-terminal (ab175108)

Overview

  • Product name
    Anti-AVL9 antibody - C-terminal
    See all AVL9 primary antibodies
  • Description
    Rabbit polyclonal to AVL9 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human AVL9 aa 619-648 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q8NBF6

  • Positive control
    • Neuro-2a cell line, Mouse brain tissue lysates. Human breast carcinoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 72 kDa.

Target

Images

  • ab175108 staining AVL9 in Saos2 human osteaosarcoma cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with TBST and blocked with 10% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/1000 in TBST) for 1 hour at 22°C. ab150092, a goat anti-rabbit Alexa Fluor® 594 (1/5000) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-AVL9 antibody - C-terminal (ab175108) at 1/1000 dilution

    Lane 1 : Neuro-2a cell lysate
    Lane 2 : Mouse brain tissue lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 72 kDa

  • Immunohistochemical analysis of formalin fixed and paraffin embedded Human breast carcinoma labeling AVL9 with ab175108 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

References

ab175108 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Sample
Human Cell (Saos2 Human Osteosarcoma)
Specification
Saos2 Human Osteosarcoma
Permeabilization
Yes - TBST
Fixative
Paraformaldehyde
Username

Jonathan Larochelle

Verified customer

Submitted Apr 14 2014

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Non-reduced Denaturing (gel 7.5%)
Sample
Dog Cell lysate - whole cell (kidney)
Specification
kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Mar 19 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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