Overview

  • Product nameAnti-AVPR V2 antibody
    See all AVPR V2 primary antibodies
  • Description
    Rabbit polyclonal to AVPR V2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chimpanzee, Gorilla, Chinese Hamster
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human AVPR V2.

  • Positive control
    • This antibody gave a positive signal in HEK293 whole cell lysate as well as the following tissue lysates: Human Fetal Kidney and Mouse Kidney.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40,43 kDa (predicted molecular weight: 40 kDa).

Target

  • FunctionReceptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate adenylate cyclase.
  • Tissue specificityKidney.
  • Involvement in diseaseDefects in AVPR2 are the cause of nephrogenic syndrome of inappropriate antidiuresis (NSIAD) [MIM:300539]. This disorder is characterized by an inability to excrete a free water load, with inappropriately concentrated urine and resultant hyponatremia, hypoosmolarity, and natriuresis.
    Defects in AVPR2 are the cause of diabetes insipidus nephrogenic X-linked (XNDI) [MIM:304800]; also known as diabetes insipidus nephrogenic type 1. XNDI is caused by the inability of the renal collecting ducts to absorb water in response to arginine vasopressin. It is characterized by excessive water drinking (polydypsia), excessive urine excretion (polyuria), persistent hypotonic urine, and hypokalemia.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Vasopressin/oxytocin receptor subfamily.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADHR antibody
    • Antidiuretic hormone receptor antibody
    • Arginine vasopressin receptor 2 antibody
    • AVP R2 antibody
    • AVPR 2 antibody
    • AVPR V2 antibody
    • AVPR2 antibody
    • DI1 antibody
    • DIR 3 antibody
    • DIR antibody
    • DIR3 antibody
    • MGC126533 antibody
    • MGC138386 antibody
    • NDI antibody
    • Nephrogenic diabetes insipidus antibody
    • Renal type arginine vasopressin receptor antibody
    • Renal-type arginine vasopressin receptor antibody
    • V2 receptor antibody
    • V2R antibody
    • V2R_HUMAN antibody
    • Vasopressin V2 antibody
    • Vasopressin V2 receptor antibody
    see all

Anti-AVPR V2 antibody images

  • All lanes : Anti-AVPR V2 antibody (ab109326) at 1 µg/ml (Milk blocking - 5%)

    Lane 1 : Kidney (Human) Tissue Lysate - fetal normal tissue (ab30204)
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 40 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 26 kDa,72 kDa,90 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • Anti-AVPR V2 antibody (ab109326) at 1 µg/ml (Milk blocking - 3%) + Kidney (Mouse) Tissue Lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 40 kDa
    Observed band size : 40 kDa


    Exposure time : 12 minutes

References for Anti-AVPR V2 antibody (ab109326)

ab109326 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab109326.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"