The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 26 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate adenylate cyclase.
Involvement in disease
Defects in AVPR2 are the cause of nephrogenic syndrome of inappropriate antidiuresis (NSIAD) [MIM:300539]. This disorder is characterized by an inability to excrete a free water load, with inappropriately concentrated urine and resultant hyponatremia, hypoosmolarity, and natriuresis. Defects in AVPR2 are the cause of diabetes insipidus nephrogenic X-linked (XNDI) [MIM:304800]; also known as diabetes insipidus nephrogenic type 1. XNDI is caused by the inability of the renal collecting ducts to absorb water in response to arginine vasopressin. It is characterized by excessive water drinking (polydypsia), excessive urine excretion (polyuria), persistent hypotonic urine, and hypokalemia.
Belongs to the G-protein coupled receptor 1 family. Vasopressin/oxytocin receptor subfamily.