Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Microbiology >> Organism >> Bacteria >> Gram negative Bacteria >> Salmonella
Overview
Product name
Anti-avrA antibody
Description
Mouse polyclonal to avrA
Tested applications
Cross reactivity
Reacts with Salmonella typhimurium.
Immunogen
Vector coding for a partial recombinant fusion protein, corresponding to amino acids 160-259 of Salmonella typhimurium avrA, [Accession number AAB83970.1] Target sequence used to make the antibody: AALEREQLPD CYFAMVELDI QRSSSECGIF SLALAKKLQL EFMNLVKIHE DNICERLCGE EPFLPSDKAD RYLPVSFYKH TQGAQRLNEY VEANPAAGSS.
AALEREQLPD CYFAMVELDI QRSSSECGIF SLALAKKLQL EFMNLVKIHE DNICERLCGE EPFLPSDKAD RYLPVSFYKH TQGAQRLNEY VEANPAAGSS
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: None
Constituents: 50% Glycerol, Whole serum
Purity
Whole antiserum
Primary antibody notes
This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al.PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
Clonality
Polyclonal
Isotype
IgG
Research areas
Microbiology >> Organism >> Bacteria >> Gram negative Bacteria >> Salmonella
-
Western blot - avrA antibody (ab52929)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab52929 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000Predicted molecular weight: 34 kDa.(This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.)
Target
Relevance
AvrA, a secreted virulence-associated effector protein, and is a member of the YopJ/Avr family of proteins. It is found in most Salmonella species. It has been reported that the AvrA protein from Salmonella typhimurium is an effector molecule that inhibits activation of the key proinflammatory NF-kB transcription factor and augments apoptosis in human epithelial cells. This activity is similar but mechanistically distinct from that described for YopJ, an AvrA homolog expressed by the bacterial pathogen Yersinia.
Cellular localization
Secreted
Alternative names
- Avirulence protein A antibody
- Putative inner membrane protein antibody
Anti-avrA antibody images:
Western blot - avrA antibody (ab52929)
All lanes : Anti-avrA antibody (ab52929) at 1/1000 dilution
Lane 1 : a total protein extract from E coli with 50ng of a tagged fusion protein of an irrelevant antigen
Lane 2 : a total protein extract from E coli with 50ng of the antigen (tagged fusion protein)
Lysates/proteins at 20 µg per lane.
Secondary
Rabbit anti-mouse IgG + IgM (H+L), HRP conjugated, at 1/5000 dilution
Predicted band size : 34 kDa
References for Anti-avrA antibody (ab52929)
ab52929 has not yet been referenced specifically in any publications.
Publishing research using ab52929? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"