Overview

  • Product nameAnti-avrA antibody
  • Description
    Mouse polyclonal to avrA
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with Salmonella typhimurium.
  • Immunogen

    Vector coding for a partial recombinant fusion protein, corresponding to amino acids 160-259 of Salmonella typhimurium avrA, [Accession number AAB83970.1] Target sequence used to make the antibody: AALEREQLPD CYFAMVELDI QRSSSECGIF SLALAKKLQL EFMNLVKIHE DNICERLCGE EPFLPSDKAD RYLPVSFYKH TQGAQRLNEY VEANPAAGSS.

  • General notes


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, Whole serum
  • PurityWhole antiserum
  • Primary antibody notesThis antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab52929 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 34 kDa. This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

Target

  • RelevanceAvrA, a secreted virulence-associated effector protein, and is a member of the YopJ/Avr family of proteins. It is found in most Salmonella species. It has been reported that the AvrA protein from Salmonella typhimurium is an effector molecule that inhibits activation of the key proinflammatory NF-kB transcription factor and augments apoptosis in human epithelial cells. This activity is similar but mechanistically distinct from that described for YopJ, an AvrA homolog expressed by the bacterial pathogen Yersinia.
  • Cellular localizationSecreted
  • Alternative names
    • Avirulence protein A antibody
    • Putative inner membrane protein antibody

Anti-avrA antibody images

  • All lanes : Anti-avrA antibody (ab52929) at 1/1000 dilution

    Lane 1 : a total protein extract from E coli with 50ng of a tagged fusion protein of an irrelevant antigen
    Lane 2 : a total protein extract from E coli with 50ng of the antigen (tagged fusion protein)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Rabbit anti-mouse IgG + IgM (H+L), HRP conjugated, at 1/5000 dilution

    Predicted band size : 34 kDa

References for Anti-avrA antibody (ab52929)

ab52929 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab52929.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"