Overview

  • Product nameAnti-Axin 1 antibody
    See all Axin 1 primary antibodies
  • Description
    Rabbit polyclonal to Axin 1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide:

    TKKGSYRYYFKKVSDEFDC

    conjugated to KLH, corresponding to amino acids near the C terminal end (815-833) of Human Axin 1

  • Positive control
    • HEK-293 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab55906 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 130 kDa.

Target

  • FunctionComponent of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling. Controls dorsoventral patterning via two opposing effects; down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway. In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B. Likely to function as a tumor suppressor. Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7. Also component of the AXIN1-HIPK2-TP53 complex which controls cell growth, apoptosis and development.
  • Tissue specificityUbiquitously expressed.
  • Involvement in diseaseHepatocellular carcinoma
    Caudal duplication anomaly
  • Sequence similaritiesContains 1 DIX domain.
    Contains 1 RGS domain.
  • DomainThe tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2.
  • Post-translational
    modifications
    Phosphorylation and dephosphorylation of AXIN1 regulates assembly and function of the beta-catenin complex. Phosphorylated by CK1 and GSK3B. Dephosphorylated by PPP1CA and PPP2CA. Phosphorylation by CK1 enhances binding of GSK3B to AXIN1.
    ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination at 'Lys-48' and subsequent activation of the Wnt signaling pathway.
    Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Sumoylation at Lys-857 and Lys-860 prevents ubiquitination and degradation. Sumoylation is required for AXIN1-mediated JNK activation. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important for nuclear accumulation during Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription.
  • Cellular localizationCytoplasm. Nucleus. Membrane. Cell membrane. MACF1 is required for its translocation to cell membrane (By similarity). On UV irradiation, translocates to the nucleus and colocalizes with DAAX (PubMed:17210684).
  • Information by UniProt
  • Database links
  • Alternative names
    • AI316800 antibody
    • AXIN antibody
    • Axin 1 antibody
    • Axin-1 antibody
    • axin1 antibody
    • AXIN1_HUMAN antibody
    • Axis inhibition protein 1 antibody
    • Axis Inhibitor 1 antibody
    • Fu antibody
    • fused antibody
    • Fused, mouse, homolog of antibody
    • hAxin antibody
    • Kb antibody
    • Ki antibody
    • kinky antibody
    • knobbly antibody
    • MGC132911 antibody
    • MGC52315 antibody
    • PPP1R49 antibody
    • Protein Fused antibody
    • Protein phosphatase 1 regulatory subunit 49 antibody
    see all

References for Anti-Axin 1 antibody (ab55906)

ab55906 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Rabbit Tissue lysate - whole (brain tissue)
Gel Running Conditions Reduced Denaturing (4-15%)
Loading amount 20 µg
Specification brain tissue
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Apr 28 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"