Overview

  • Product nameAnti-Axin 1 antibody
    See all Axin 1 primary antibodies
  • Description
    Mouse monoclonal to Axin 1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: ISRHRRTGHG SSGTRKPQPH ENSRPLSLEH PWAGPQLRTS VQPSHLFIQD PTMPPHPAPN PLTQLEEARR RLEEEEKRAS RAPSKQRYVQ EVMRRGRA, corresponding to amino acids 643-741 of Human Axin 1

Properties

Applications

Our Abpromise guarantee covers the use of ab56475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionComponent of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling. Controls dorsoventral patterning via two opposing effects; down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway. In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B. Likely to function as a tumor suppressor. Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7. Also component of the AXIN1-HIPK2-TP53 complex which controls cell growth, apoptosis and development.
    • Tissue specificityUbiquitously expressed.
    • Involvement in diseaseHepatocellular carcinoma
      Caudal duplication anomaly
    • Sequence similaritiesContains 1 DIX domain.
      Contains 1 RGS domain.
    • DomainThe tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2.
    • Post-translational
      modifications
      Phosphorylation and dephosphorylation of AXIN1 regulates assembly and function of the beta-catenin complex. Phosphorylated by CK1 and GSK3B. Dephosphorylated by PPP1CA and PPP2CA. Phosphorylation by CK1 enhances binding of GSK3B to AXIN1.
      ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination at 'Lys-48' and subsequent activation of the Wnt signaling pathway.
      Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Sumoylation at Lys-857 and Lys-860 prevents ubiquitination and degradation. Sumoylation is required for AXIN1-mediated JNK activation. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important for nuclear accumulation during Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription.
    • Cellular localizationCytoplasm. Nucleus. Membrane. Cell membrane. MACF1 is required for its translocation to cell membrane (By similarity). On UV irradiation, translocates to the nucleus and colocalizes with DAAX (PubMed:17210684).
    • Information by UniProt
    • Database links
    • Alternative names
      • AI316800 antibody
      • AXIN antibody
      • Axin 1 antibody
      • Axin-1 antibody
      • axin1 antibody
      • AXIN1_HUMAN antibody
      • Axis inhibition protein 1 antibody
      • Axis Inhibitor 1 antibody
      • Fu antibody
      • fused antibody
      • Fused, mouse, homolog of antibody
      • hAxin antibody
      • Kb antibody
      • Ki antibody
      • kinky antibody
      • knobbly antibody
      • MGC132911 antibody
      • MGC52315 antibody
      • PPP1R49 antibody
      • Protein Fused antibody
      • Protein phosphatase 1 regulatory subunit 49 antibody
      see all

    Anti-Axin 1 antibody images

    • Western blot against tagged recombinant protein immunogen using ab56475 Axin 1 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa

    References for Anti-Axin 1 antibody (ab56475)

    ab56475 has not yet been referenced specifically in any publications.

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