Overview

  • Product name
  • Description
    Rabbit polyclonal to Axin 1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide contain a sequence corresponding to a region within amino acids 800-862 of Human Axin 1 (NP_003493)

  • Positive control
    • A431, H1299 lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab96856 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 96 kDa.

Target

  • Function
    Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling. Controls dorsoventral patterning via two opposing effects; down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway. In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B. Likely to function as a tumor suppressor. Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7. Also component of the AXIN1-HIPK2-TP53 complex which controls cell growth, apoptosis and development.
  • Tissue specificity
    Ubiquitously expressed.
  • Involvement in disease
    Hepatocellular carcinoma
    Caudal duplication anomaly
  • Sequence similarities
    Contains 1 DIX domain.
    Contains 1 RGS domain.
  • Domain
    The tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2.
  • Post-translational
    modifications
    Phosphorylation and dephosphorylation of AXIN1 regulates assembly and function of the beta-catenin complex. Phosphorylated by CK1 and GSK3B. Dephosphorylated by PPP1CA and PPP2CA. Phosphorylation by CK1 enhances binding of GSK3B to AXIN1.
    ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination at 'Lys-48' and subsequent activation of the Wnt signaling pathway.
    Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Sumoylation at Lys-857 and Lys-860 prevents ubiquitination and degradation. Sumoylation is required for AXIN1-mediated JNK activation. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important for nuclear accumulation during Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription.
  • Cellular localization
    Cytoplasm. Nucleus. Membrane. Cell membrane. MACF1 is required for its translocation to cell membrane (By similarity). On UV irradiation, translocates to the nucleus and colocalizes with DAAX (PubMed:17210684).
  • Information by UniProt
  • Database links
  • Alternative names
    • AI316800 antibody
    • AXIN antibody
    • Axin 1 antibody
    • Axin-1 antibody
    • axin1 antibody
    • AXIN1_HUMAN antibody
    • Axis inhibition protein 1 antibody
    • Axis Inhibitor 1 antibody
    • Fu antibody
    • fused antibody
    • Fused, mouse, homolog of antibody
    • hAxin antibody
    • Kb antibody
    • Ki antibody
    • kinky antibody
    • knobbly antibody
    • MGC132911 antibody
    • MGC52315 antibody
    • PPP1R49 antibody
    • Protein Fused antibody
    • Protein phosphatase 1 regulatory subunit 49 antibody
    see all

Images

  • All lanes : Anti-Axin 1 antibody (ab96856) at 1/1500 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 96 kDa

References

ab96856 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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