Overview

  • Product nameAnti-Axl antibody
    See all Axl primary antibodies
  • Description
    Mouse monoclonal to Axl
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human, African Green Monkey
  • Immunogen

    Recombinant fragment, corresponding to amino acids 30-140 of Human Axl

Properties

Applications

Our Abpromise guarantee covers the use of ab54803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMay function as a signal transducer between specific cell types of mesodermal origin. In case of filovirus infection, seems to function as a cell entry factor.
    • Tissue specificityHighly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue.
    • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
      Contains 2 fibronectin type-III domains.
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 protein kinase domain.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Adhesion related kinase antibody
      • AI323647 antibody
      • Ark antibody
      • Axl antibody
      • AXL oncogene antibody
      • AXL receptor tyrosine kinase antibody
      • AXL transforming gene antibody
      • AXL transforming sequence/gene antibody
      • EC 2.7.10.1 antibody
      • JTK11 antibody
      • Oncogene AXL antibody
      • Tyro7 antibody
      • Tyrosine protein kinase receptor UFO antibody
      • Tyrosine-protein kinase receptor UFO antibody
      • UFO antibody
      • UFO_HUMAN antibody
      see all

    Anti-Axl antibody images



    • Predicted band size : 97 kDa
    • All lanes : Anti-Axl antibody (ab54803) at 1/2000 dilution (in PBS + 3% BSA + 0.02% sodium azide for 16 hours at 4°C)

      Lane 1 : Whole cell lysate of MCF7 infected with human Axl expressing lentivirus (high expression)
      Lane 2 : Whole cell lysate of MCF7 infected with human Axl expressing lentivirus (low expression)
      Lane 3 : Whole cell lysate of non-infected MCF7

      Lysates/proteins at 10 µg per lane.

      Secondary
      An HRP-conjugated Donkey anti-mouse polyclonal at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 97 kDa
      Observed band size : ~100 kDa (why is the actual band size different from the predicted?)


      Exposure time : 30 minutes

      This image is courtesy of an Abreview submitted by Nir Drayman

      See Abreview

    References for Anti-Axl antibody (ab54803)

    ab54803 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunoprecipitation
    Sample African Green Monkey Cell lysate - whole cell (CV1)
    Total protein in input 1e+007 cells
    Specification CV1
    Immuno-precipitation step Protein A/G
    Username

    Mr. Nir Drayman

    Verified customer

    Submitted May 11 2011

    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (MCF7)
    Loading amount 10 µg
    Specification MCF7
    Treatment Infected with lentivirus over expressing human Axl
    Gel Running Conditions Reduced Denaturing (4-12% PA gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Mr. Nir Drayman

    Verified customer

    Submitted Feb 01 2011

    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application Flow Cytometry
    Sample African Green Monkey Cell (CV1 cells)
    Specification CV1 cells
    Preparation Cell harvesting/tissue preparation method: harvest by addition of 0.05% EDTA for 2 minutes.
    Sample buffer: PBS
    Fixation no fixaion
    Permeabilization No
    Gating Strategy cells stained with secondary antibody only were used for gating
    Username

    Mr. Nir Drayman

    Verified customer

    Submitted Jan 13 2011

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample African Green Monkey Cell (CV1 cells)
    Specification CV1 cells
    Fixative Paraformaldehyde
    Permeabilization No
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Mr. Nir Drayman

    Verified customer

    Submitted Jan 04 2011

    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample African Green Monkey Cell lysate - whole cell (CV1 cells)
    Loading amount 10 µg
    Specification CV1 cells
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Mr. Nir Drayman

    Verified customer

    Submitted Jan 03 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"