Recombinant fragment, corresponding to amino acids 30-140 of Human Axl
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
All lanes : Anti-Axl antibody (ab54803) at 1/2000 dilution (in PBS + 3% BSA + 0.02% sodium azide for 16 hours at 4°C)
Lane 1 : Whole cell lysate of MCF7 infected with human Axl expressing lentivirus (high expression) Lane 2 : Whole cell lysate of MCF7 infected with human Axl expressing lentivirus (low expression) Lane 3 : Whole cell lysate of non-infected MCF7
Lysates/proteins at 10 µg per lane.
Secondary An HRP-conjugated Donkey anti-mouse polyclonal at 1/5000 dilution Developed using the ECL technique