Overview

  • Product name
  • Description
    Mouse monoclonal to Axl
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human, African green monkey
  • Immunogen

    Recombinant fragment, corresponding to amino acids 30-140 of Human Axl

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

Properties

Applications

Our Abpromise guarantee covers the use of ab54803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: Use at a concentration of 1-5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      May function as a signal transducer between specific cell types of mesodermal origin. In case of filovirus infection, seems to function as a cell entry factor.
    • Tissue specificity
      Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue.
    • Sequence similarities
      Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
      Contains 2 fibronectin type-III domains.
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 protein kinase domain.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Adhesion related kinase antibody
      • AI323647 antibody
      • Ark antibody
      • Axl antibody
      • AXL oncogene antibody
      • AXL receptor tyrosine kinase antibody
      • AXL transforming gene antibody
      • AXL transforming sequence/gene antibody
      • EC 2.7.10.1 antibody
      • JTK11 antibody
      • Oncogene AXL antibody
      • Tyro7 antibody
      • Tyrosine protein kinase receptor UFO antibody
      • Tyrosine-protein kinase receptor UFO antibody
      • UFO antibody
      • UFO_HUMAN antibody
      see all

    Images



    • Predicted band size : 97 kDa
    • All lanes : Anti-Axl antibody (ab54803) at 1/2000 dilution (in PBS + 3% BSA + 0.02% sodium azide for 16 hours at 4°C)

      Lane 1 : Whole cell lysate of MCF7 infected with human Axl expressing lentivirus (high expression)
      Lane 2 : Whole cell lysate of MCF7 infected with human Axl expressing lentivirus (low expression)
      Lane 3 : Whole cell lysate of non-infected MCF7

      Lysates/proteins at 10 µg per lane.

      Secondary
      An HRP-conjugated Donkey anti-mouse polyclonal at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 97 kDa
      Observed band size : ~100 kDa (why is the actual band size different from the predicted?)


      Exposure time : 30 minutes

      This image is courtesy of an Abreview submitted by Nir Drayman

      See Abreview

    References

    ab54803 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunoprecipitation
    Sample
    African Green Monkey Cell lysate - whole cell (CV1)
    Total protein in input
    1e+007 cells
    Specification
    CV1
    Immuno-precipitation step
    Protein A/G
    Username

    Mr. Nir Drayman

    Verified customer

    Submitted May 11 2011

    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (MCF7)
    Loading amount
    10 µg
    Specification
    MCF7
    Treatment
    Infected with lentivirus over expressing human Axl
    Gel Running Conditions
    Reduced Denaturing (4-12% PA gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Mr. Nir Drayman

    Verified customer

    Submitted Feb 01 2011

    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Flow Cytometry
    Sample
    African Green Monkey Cell (CV1 cells)
    Specification
    CV1 cells
    Preparation
    Cell harvesting/tissue preparation method: harvest by addition of 0.05% EDTA for 2 minutes.
    Sample buffer: PBS
    Fixation
    no fixaion
    Permeabilization
    No
    Gating Strategy
    cells stained with secondary antibody only were used for gating
    Username

    Mr. Nir Drayman

    Verified customer

    Submitted Jan 13 2011

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    African Green Monkey Cell (CV1 cells)
    Specification
    CV1 cells
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Mr. Nir Drayman

    Verified customer

    Submitted Jan 04 2011

    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    African Green Monkey Cell lysate - whole cell (CV1 cells)
    Loading amount
    10 µg
    Specification
    CV1 cells
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Mr. Nir Drayman

    Verified customer

    Submitted Jan 03 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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