Anti-Axl antibody [MM0098-2N33] (ab89224)

Overview

  • Product name
    Anti-Axl antibody [MM0098-2N33]
    See all Axl primary antibodies
  • Description
    Mouse monoclonal [MM0098-2N33] to Axl
  • Tested applications
    Suitable for: IP, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human, African green monkey
  • Immunogen

    Human recombinant Axl (extracellular region).

Properties

Applications

Our Abpromise guarantee covers the use of ab89224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt 1/50 - 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    May function as a signal transducer between specific cell types of mesodermal origin. In case of filovirus infection, seems to function as a cell entry factor.
  • Tissue specificity
    Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adhesion related kinase antibody
    • AI323647 antibody
    • Ark antibody
    • Axl antibody
    • AXL oncogene antibody
    • AXL receptor tyrosine kinase antibody
    • AXL transforming gene antibody
    • AXL transforming sequence/gene antibody
    • EC 2.7.10.1 antibody
    • JTK11 antibody
    • Oncogene AXL antibody
    • Tyro7 antibody
    • Tyrosine protein kinase receptor UFO antibody
    • Tyrosine-protein kinase receptor UFO antibody
    • UFO antibody
    • UFO_HUMAN antibody
    see all

Anti-Axl antibody [MM0098-2N33] images

  • ab89224 used in Flow Cytometry.
    Cells were trypsinized for 1 minute at room temperature and wahed once in cold PBS. They were stained with ab89224 diluted 1/200 in 5% BSA/PBS for 45 minutes on ice, washed and incbuated with ab98794 at a 1/200 dilution. Cells were filtered and read in a FACScan using the FL1 sensor.
  • ab89224 staining Axl in african green monkey CV1 cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in 4% paraformaldehyde and then blocked using 5% serum for 1 hour at 22°C. Samples were then incubated with primary antibody at 1/100 for 16 hours at 4°C. The secondary antibody used was a donkey anti-mouse IgG conjugated to Alexa Fluor® 546 (red) used at a 1/400 dilution. DAPI was used to counterstain nuclei.

References for Anti-Axl antibody [MM0098-2N33] (ab89224)

ab89224 has not yet been referenced specifically in any publications.

Product Wall

I can confirm that this product does not contain any azide. It only contains PBS.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (HeLa)
Specification
HeLa
Preparation
Cell harvesting/tissue preparation method: Cells were collected by trypsinization
Sample buffer: PBS
Fixation
no fixation
Permeabilization
No
Username

Mr. Nir Drayman

Verified customer

Submitted Nov 23 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
African Green Monkey Cell (CV1)
Specification
CV1
Fixation
no fixation
Permeabilization
No
Username

Mr. Nir Drayman

Verified customer

Submitted May 28 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
African Green Monkey Cell lysate - whole cell (CV1 cells)
Total protein in input
1e+007 cells
Specification
CV1 cells
Immuno-precipitation step
Protein A/G
Username

Mr. Nir Drayman

Verified customer

Submitted May 11 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
African Green Monkey Cell (CV1)
Specification
CV1
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Mr. Nir Drayman

Verified customer

Submitted Apr 21 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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